脑舒胶囊对阿尔茨海默病大鼠神经炎症损伤的保护

Protective effect of Naoshu capsule on neuroinflammatory injury in rat model of Alzheimer's disease

目的：研究脑舒胶囊对阿尔茨海默病（AD）大鼠神经炎症损伤的保护。方法：双侧脑室注射Aβ25~35复制AD大鼠模型;放射免疫分析法检测血清及海马组织IL-1β和IL-6的含量;RT-PCR法测定海马IL-6和IL-1βmRNA表达;免疫组织化学（SABC）法检测大鼠海马星形胶质细胞原纤维酸性蛋白（GFAP）的阳性细胞表达。结果：与假手术组比较,模型组大鼠血清及海马组织中IL-1β和IL-6含量明显增加（P〈0.05）;海马星形胶质细胞GFAP和海马IL-6,IL-1βmRNA表达显著上调（P〈0.01）。与模型组比较,0.72g.kg-1脑舒胶囊可降低血清、海马组织IL-1β和IL-6含量（P〈0.05）和海马星形胶质细胞GFAP阳性和海马IL-6,IL-1βmRNA的表达（P〈0.05）。结论：脑舒胶囊可通过抑制星形胶质细胞的过度激活,从而抑制中枢慢性炎症级联反应对海马神经元的损伤。

Objective： To investigate the protective effect of Naoshu capsule on neuroinflammatory injury in rat model of Alzheimer＇s disease（AD）.Methods： AD model was induced by injecting β-amyloid25~35（Aβ25~35） into the bilateral lateral ventricles of rats.The concentrations of interleukin-1β（IL-1β） and interleukin-6（IL-6） in the serum and hippocampus were determined by radioimmunoassay.The mRNA expression of IL-6 and IL-1β was detected by RT-PCR,and glial fibrillary acidic protein（GFAP） in hippocampal astrocytes was determined by immunostaining（SABC）.Results： The concentrations of IL-1β and IL-6 in the serum and hippocampus increased（P0.05）,and the expression of GFAP and IL-6/IL-1β mRNA in the hippocampus increased（P0.01） after Aβ25~35 injection.Naoshu capsule（0.72 g·kg-1） significantly decreased the concentrations of IL-1β and IL-6 in the serum and hippocampus（P0.05）,and inhibited the expression of GFAP and IL-6/IL-1β mRNA（P0.05） in AD model rats.Conclusions： Naoshu capsule can protect hippocampal neurons by inhibiting excessive activation of astrocytes and chronic neuroinflammation cascade reactions.