摘要
海风藤团为血小板激活因子PAF的特异性受体桔抗剂。用含20μg·mL-1海风藤酮的培养液培养兔2细胞胚胎144h，发育到等于或小于16细胞的胚胎比例为66．9％，对照组为13．5％（P＜0．025）。发育到襄胚和孵化囊胚的比例，对照组为39．9％，而试验组发育到囊胚的比例为2．7％（P＜0．001），没有胚胎发育到孵化囊胚阶段。试验表明海风藤团对胚胎的体外发育有阻滞作用。试验还表明，兔胚胎可以释放PAF，海风藤国能明显抑制免胚胎培养液所引起的去牌鼠血小板数量下降的PAF效应。
?Kadsurenone is a competitive antagonist of PAF receptor. Rabbit 2-cell embryoeswere cultured in the culture medium containing 20 μg.mL-1 kadsurenone for 144 h. 66. 9%embryoes developed to 16-cell or smaller than 16-cell stage. It was 13. 5% (P<0. 025) incontrol group. 39. 9% embryoes developed to blastocyst and hatched blastocyst stage in con-trol group- but only 2. 7% (P< 0. 001) embryoes developed to blastocyst stage in testgroup, none of them developed to hatched blastocyst stage. It showed that kadsurenone hadan inhibitory effect on embryo development in vitro. The result also showed that rabbit em-bryo could release PAF and kadsurenone could distinctly inhibit the PAF effect of splenec-tomized mice platelet number decrease causcd by rabbit embryo cultured medium.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
1999年第1期15-19,共5页
Journal of China Agricultural University
基金
国家自然科学基金!39270516
关键词
兔胚
海风藤酮
血小板激活因子
发育
rabbit embryo
kadsurenone
platelet activating factor(PAF)
