棉酚脱毒微生物的筛选及其脱毒效果的研究

THE SCREENING OF SHEDDING GOSSYPOL TOXICITY AND TOXICANT-SHEDDING RESUITS

经反复发酵脱毒试验，筛选出了一株棉酚脱毒菌（Ｓ９６０６）。试验结果证明，该菌能将含棉酚在０．０４５％～０．１８％范围内的各种棉籽饼（粕）一次性脱毒，使游离棉酚残留量在０．０２６％以下，脱毒率随棉酚含量的升高而升高（４９％～８５．３％）。脱毒后的棉籽蛋白中有６种必需氨基酸（蛋氨酸、赖氨酸、缬氨酸、苏氨酸、亮氨酸、异亮氨酸）的含量增加，其中蛋氨酸提高了３５．７％～４０％，赖氨酸提高了１９．４％～２７．６％，同时精氨酸减少２０％左右，使赖氨酸与精氨酸之比接近正常的吸收比值。发酵脱毒后的棉籽饼（粕）质量有了明显的提高。

After the expeximents of fermenting and shedding toxicant time and again, a kind of fungus(S 9606 )of shedding gossypol toxicity was screened.The results of the experiments prove that the fungus can shed gossypol in cottonseed meal whose gossypol content is 0.045%～0.18% and decrease its free gossypol content to 0.026% and even below.The gossypol toxicity shedding rate is 49%～85.3%.After the cottonseed meal is fermentated and shedding gossypol toxicity,the contents of six essential amino acids (Met、Lys、Val、Thr、Len、Ile)has all increased.Among them Methinoine increases by 35.7%～40%,Lysine increases by 19.4%～27.6%,but Arginine decreases by about 20%,the ratio of lys and Arg.Approaches normal Absorption specific ratio.After the cottonseed meal is fermentated,the quality is improved strikingly.