用PCR-RFLP技术鉴别猪的氟烷基因型

IDENTIFICATION ON PORCINE HALOTHANE GENOTYPES BY POLYMEROSE CHAIN REACTION

用ＰＣＲ－ＲＦＬＰ技术对乐山市种畜场长白猪选育基础群的氟烷基因型进行了检测，查清了Ｈａｌｎ基因在该群体中的分布，Ｈａｌｎ基因的频率为１８０５６％。同时对影响ＰＣＲ的几个主要因素进行了初步研究。

Porcine Stress Syndrome(PSS) is controlled by Halothanegene located on porcine No.6 chromosome. Identification of the porcineHalothane genotypes was established by PCR-RFLP. The Halothane genefragment containing the mutation point was amplified and the PCR product was incubated with restiction endonuclease HhaI, then genotypes were detected. And several factors that effected PCR are analysised.