药物诱发豚鼠胆囊炎的病理学及酶组化研究

THE STUDY OF PATHOLOGY AND ENZYMIC HISTOCHEMISTRY OF CHOLECYSTITIS CAUSED BY DIFFERENT DRUGS

以酶病理组织化学方法研究了林可霉素、石胆酸、硫氢酸α萘酯和月桂酸红霉素酯四种药物诱发豚鼠胆囊炎和胆结石形成过程中，酶、粘多糖及胆汁成份的变化。结果表明：四种药物都能诱发豚鼠胆囊炎，内源性βＧＣＤ酶含量显著升高且与胆囊炎症程度明显相关，胆汁βＧＣＤ酶与ＵＣＢ／ＴＢ比值显著正相关；βＧＣＤ酶和Ｃａ２＋的关系与成石时间有关而各组有所差异。胆囊和肝脏ＡＣＰ、ＡＫＰ和Ｇ６Ｐ活性也有明显变化。本文进一步证实内源性βＧＣＤ和酸性粘多糖在胆色素结石形成中有关键作用。

The aim of the present study is to establish the cholecystitis model in guinea pig which induced by four different drugs:Lithocholic acid,Lincomycin,α naphthylisothiocyanate,and Erythromycin estolate.Through our research by using histopathology and enzymic histochemistery techniques,we found all of the drugs could induce cholecystitis to a different degree,which general characteristic was the rise of intrastital β glucuronidase and acid mucopolysaccharides in bile and gallbladder tissues.It is concluded that β glucuronidase is interrelated to the severity of cholecystitis and to UCB/TB in bile of test group.The Ca 2+ of bile was affected by the time course of gallstone formation.