恶性纤维组织细胞瘤和血管内皮瘤的免疫组织化学研究

AN IMMUNOHISTOCHEMICAL STUDY ON THE MALIGNANT FIBROHISTIOCYTOMA AND THE HEMANGIOENDOTHELIOMA

应用免疫过氧化酶技术研究了恶性纤维组织细胞瘤、血管内皮瘤和血管母细胞瘤。6例恶性纤维组织细胞瘤的A_1抗糜蛋白酶均为阳性。有6例原诊断为纤维肉瘤,但A_1抗糜蛋白酶亦为阳性,复查切片虽无典型的车辐状排列,但可见典型的巨细胞,泡沫细胞及多量炎细胞浸润,因此诊断为恶性纤维组织细胞瘤。7例血管内皮瘤均为第Ⅷ因子相关抗原阳性,其中2例为分化较差的血管内皮瘤。表明A_1抗糜蛋白酶和第Ⅷ因子相关抗原分别是恶性纤维组织细胞瘤和血管内皮瘤有价值的标记物。8例小脑血管母细胞瘤的第Ⅷ因子相关抗原呈阴性反应。表明血管母细胞瘤可能来源于血管外皮细胞。

Malignant fibrohistiocytoma(MFH),hemangioendotheliomaand hemangioblastoma were studied by means of indirect immunoperoxidasetechnique,Six cases of MFH were positive stained for Al-antichymotry-psin,Six previously diagnosed fibrosarcomas had positive reaction forAl-antichymotrypsin.Reviewing the slides of these cases made us believethat the 6 fibrosarcomas were MFHs even though they lacked the typic histological appearance of histiocytic origin.Malignant giant cells andfoamy cells were notable on the sildes.Seven hemangioendotheliomas werepositive stained for Ⅷ related antigen(Factor Ⅷ),including 2 poorly differentiated cases,The results indicate that Al-antichymotrysin andFactor Ⅷ are useful and significant markers for fibrohistiocytoma and he-mangioendothelioma respectively.All the 8 hemangioblastomas were nega- tive stained for Factor Ⅷ,which suggests that this tumor is originatedfrom hemangiopericyte.