用RT-PCR对mRNA进行定量分析

Quantitative analysis of mRNA with RT-PCR

编码蛋白质的mRNA通过反转录酶的作用，将mR－NA反转录成cDNA，再通过PCR扩增，DNA产物得到指数形式的增加．在一定循环数内，PCR产物的量与其模板cD－NA，也就是相应mRNA的浓度有关．PCR产物电泳后掺入溴化乙锭，可通过扫描荧光强度来确定PCR产物的量．通过优化实验条件，可计算出所检测的mRNA的相对含量．

mRNA of encoding protein was reverse transcripted to be cDNA by polymerase chain reaction (PCR). With the enhancement of PCR,DNA production increased to exponential multiple. And the PCR production was related to the CDNA template or the concentration of mRNA during optimum cycles. After electrophoresis, stained ethidium bromide (EB) into PCR products,the quantity of PCR product was determined by scanning of fluorescent intensity. The relative concentration of mRNA can be calculated by optimizing the experimental condition.