结肠癌细胞株、癌组织及正常结肠粘膜中P^(16)基因异常甲基化率的差异

The Difference of P^(16) Gene's Aberrant Methylation Rate Between Colon Cancer and Normal Colonic Mucosa

本文采用Southernblot法鉴定了结肠癌细胞株、癌组织及正常结肠粘膜组织中P16基因的甲基化情况。结果在6株结肠癌细胞株中有5株(833%)P16基因CpG岛呈异常甲基化状态,在18例结肠癌组织中13例(722%)呈异常甲基化状态。与之相对,18例正常结肠粘膜组织中仅5例(278%)表现为异常甲基化,提示在结肠癌中P16基因因异常甲基化而失活,这为结肠癌的基因治疗提供了新策略、新目标。

The methylation rate of P 16 gene in colon cancer cell lines,colonic cancer tissue and normal colonic mucosa were examined by Southern blot method.The data showed that aberrant methylation of 5'CpG island of P 16 gene was observed in 5 of 6(83 3%) colon cancer cell lines,13 of 18(72 2%) colon cancer tissue.In contrast,only 5 of 18(27 8%) normal colonic mucosa showed aberrant methylation of the 5'CpG island of P 16 gene.The results suggest that P 16 gene in colon cancer was inactivated by aberrant methylation.This can provide a new tactics and a new target for colon's gene therapy.