三硝酸铈致大鼠肝脏脂质过氧化损伤

Study on the hepatic toxicity of cerous nitrate in rats

ＳＤ成年大鼠随机分为２组，每组３０只，另设对照组（６０只）。一次性腹腔注射１５０ｍｇ／ｋｇＣｅ（ＮＯ３）３，分别于３、６、１２、２４、４８ｈ处死，测定其肝脏蛋白质浓度、ＭＤＡ、ＧＳＨ含量及ＳＯＤ、ＣＡＴ、ＧＳＨ、Ｐｘ、ＧＳＨ、ＳＴ酶的活性。结果显示，注入Ｃｅ（ＮＯ３）３以后，大鼠肝脏内蛋白质浓度、ＭＤＡ含量明显增加，而ＧＳＨ含量及ＳＯＤ、ＧＡＴ、ＧＳＨ－ＰＸ、ＧＳＨ－ＳＴ酶的活性则显著降低。提示Ｃｅ（ＮＯ３）３进入大鼠体内后，早期能够引起肝脏脂质过氧化损伤，使大鼠肝脏抗氧化能力、抗诱变能力降低，估计脂质过氧化损伤可能是由于Ｃｅ３＋与ＧＳＨ作用导致ＧＳＨ耗竭所致。

Rats were injected with a single dose of cerous nitrate Ce(NO 3) 3(150mg/kg)intra peritoneally and killed at 3,6,12,24 and 48 hours later.The results showed that the concentrations of protein and malondialdehyde (MDA) in liver increased,but the concentration of glutathione (GSH) and the activity of superoxide dismutase (SOD),catalase (CAT),glutathione peroxidase (GSH Px) and glutathione sulfatransferase (GSH ST) decreased after Ce 3+ administration.The results suggest that lipid peroxidation in liver may be an early consequence of Ce 3+ exposure and the decrease of GSH might be considered as the cause of lipid peroxidation.