脱氧雪腐镰刀菌烯醇、黄曲霉毒素G_1对体外培养人外周血淋巴细胞凋亡影响的研究

Effect of deoxynivalenol and aflatoxin G 1 on apoptosis of human blood lymphocytes in vitro

以细胞培养、流式细胞仪ＤＮＡ含量分析及ＤＮＡ琼脂糖凝胶电泳方法研究了我国食管癌高发区磁县居民粮食中优势污染霉菌毒素脱氧雪腐镰刀菌烯醇（ＤＯＮ）和黄曲霉毒素Ｇ１（ＡＦＧ１）对人淋巴细胞凋亡的影响。流式细胞术（ＦＣＭ）检测结果显示，ＤＯＮ、ＡＦＧ１处理组淋巴细胞出现了典型的亚二倍体凋亡细胞峰，凋亡百分率与毒素作用时间（ＤＯＮ：２～７２小时；ＡＦＧ１：２～２４小时）及剂量（ＤＯＮ：５０～２０００μｇ／Ｌ；ＡＦＧ１：３．１２～２０００μｇ／Ｌ）呈正相关关系。ＤＮＡ琼脂糖凝胶电泳结果表明，ＤＯＮ（１０００μｇ／Ｌ）、ＡＦＧ１（１０００μｇ／Ｌ）处理２４小时淋巴细胞出现特征性的ＤＮＡ＂Ｌａｄｄｅｒ＂条带。因此表明，ＤＯＮ、ＡＦＧ１可诱导和促进体外培养的人外周血淋巴细胞发生凋亡。

Effect of deoxynivalenol (DON) and aflatoxin G 1(AFG 1) on human blood lymphocytes in culture were studied with flow cytometric DNA content analyses and DNA agarose gel electrophoresis. DON and AFG 1 are two of the predominant mycotoxins contaminating foodstuffs or residents in a high incidence of esophageal cancer in Cixian County, Hebei province. A typical sub diploid apoptosis peak was demonstrated in lymphocytes treated with DON and AFG 1. A significant dose effect response and time effect correlation could be found between apoptosis rates and mycotoxin concentrations (DON:50～2000 μg/L and AFG 1:3 12～2000 μg/L) and the treated time (DON:2～72 hours and AFG 1:2～24 hours). The lymphocytes treated with DON and AFG 1 showed characteristic ‘ladder’ pattern in agarose gel electrophoresis. All results confirmed that DON and AFG 1 could induce and accelerate apoptosis in human peripheral blood lymphocytes.