酸枣、山楂、葡萄的体外抗氧化作用研究

The antioxidant activity of wild jujubi,crataegus and grape in vitro

采用Ｆｅ２＋－半胱氨酸诱发大鼠肝微粒体生成丙二醛（ＭＤＡ）和用过氧化叔丁醇（ｔｅｒｔ－ｂｕｔｙｌｈｙ－ｄｒｏｐｅｒｏｘｉｄｅ）损伤大鼠红细胞生成ＭＤＡ两种损伤体系，分别观察酸枣、山楂、葡萄３种果汁的体外抗脂质过氧化作用。结果表明，在２．５～３．３ｍｇ／ｍｌ浓度下，葡萄、山楂和酸枣３种果汁对Ｆｅ２＋－半胱氨酸诱发大鼠肝微粒体ＭＤＡ生成的抑制率分别达到４６．２％、９８．３％和９９．１％，这３种果汁对过氧化叔丁醇所致大鼠红细胞生成ＭＤＡ的抑制率分别为葡萄汁（５５．６ｍｇ／ｍｌ）５８．５％、山楂汁（１３．９ｍｇ／ｍｌ）３８．７％和酸枣汁（８．３ｍｇ／ｍｌ）３８．７％。采用ＥＳＲ测定３种果汁对Ｏ－２和ＯＨ自由基的清除作用。结果表明高浓度时，葡萄汁（１３４ｍｇ／ｍｌ）、山楂汁（５０ｍｇ／ｍｌ）和酸枣汁（５０ｍｇ／ｍｌ）对Ｏ－２的清除率分别为７４．７％、８５．８％和８４．０％，对ＯＨ自由基的清除率分别为８６．３％、９７．８％和９６．７％。上述结果表明，这３种果汁具有较强的抗氧化作用。

Two oxidative damaging systems,Fe 2+ Cys and tert butyl hydroperoxide,were used to induce the production of malondialdehyde (MDA) in rat liver microsome and erythrocytes.The inhibition rate of wild jujubi,crataegus and grape juice at the concentration of 2 5～3 3mg/ml to Fe 2+ Cys system on the production of MDA were 46 2%,98 3% and 99 1% respectively.The inhibition rate of jujubi (8 3mg/ml),crataegus (13 9 mg/ml) and grape (55 6 mg/ml) to tert butyl hydroperoxide were 38 7%,38 7% and 58 5% respectively.The capability of scavenging O -· 2 and ·OH was measured by ESR technique.It was found that the capability of these juices to eliminate O -· 2 and ·OH generated by the xanthine/xanthine oxidase system and H 2O 2 FeSO 4 system was strong.The elimination rate of jujubi (50 mg/ml),crataegus (50 mg/ml) and grape (134 mg/ml) to O -· 2 were 84 0%,85 8% and 74 7% respectively and the rate to · OH were 96 7%,97 8% and 86 3% respectively.The results indicated that these three fruits bear antioxidant activity.