摘要
探讨c-myc基因表达在雏鸡基底乳头(basilar papilla, BP)庆大霉素损伤后听毛细胞再生过程中的意义。方法:应用原位杂交和免疫组化技术检测庆大霉素停药后第 1,3,7,14,21和 28 d时BP损伤区域c-myc mRNA和 c-myc蛋白表达情况。结果:BP修复过程中c-myc mRNA和 c-myc蛋白表达水平明显升高,修复 7d时表达水平达高峰,修复 14 d后BP中c-myc基因表达水平开始下降。结论:提示c-myc基因表达是毛细胞再生过程中的一个重要分子事件,可能与前体细胞增殖有关。
Objective:To study the effect of c-myc gene expression during hair cell (HC) regeneration in the chick basilar papilla (BP) following getamicin ototoxicity. Method: c-myc mRNA and its product (c-myc protein ) expression in the damaged region was histologicolly analyzed by in situ hybridization and immunohistochemistry. Thirty-six animals received one subcutaneous injection of gentamicin sulfate (5 0 mg /kg ) daily for ten days. The animals were allowed to survive 1, 3, 7, 14, 21 or 28 days (n=6 in each group) before sacrifice and preparation for both in situ hybridization and immunohistochemistry. Twelve animals were sacrificed as control for each groups (n=2). Result:The results showed that the supporting cells and cells in the lumenal nuclear layer within the damaged region were strongly labeled for c-myc transcripts and its protein. Seven days after last injection, the highest level of c-myc mRNA and c-myc protein expression was observed in the injured sensory epithelium. C-myc gene expression began to decline from fourteen days post injection. High level expression of c-myc gene was discussed in relation to the time and location of HC regeneration. Conclusion: The results indicate that c-mys gene expression may be an important molecular event in HC regeneration and may play a role in regulating progenitor cells proliferation.
出处
《临床耳鼻咽喉科杂志》
CSCD
1999年第4期173-175,共3页
Journal of Clinical Otorhinolaryngology