bcl-2基因表达在人膀胱癌多药耐药现象中的意义

The inhibition of BIU 87 cell proliferation by liposome conjugated antisense oligonucleotides targeting the mRNA encoding proliferating cell nuclear antigen

目的探讨ｂｃｌ２基因在人膀胱癌多药耐药中的作用。方法应用原位ＤＮＡ末端转移酶标记法（ＴＵＮＥＬ）、免疫细胞化学及逆转录多聚酶链式反应（ＲＴＰＣＲ）技术，分别对阿霉素诱导的人膀胱癌耐药细胞株（ＢＩＵ８７／ＡＤＭ）及敏感细胞株（ＢＩＵ８７）的细胞凋亡及其ｂｃｌ２基因表达进行研究。结果（１）在相同条件下，与敏感细胞株相比，耐药细胞株中细胞凋亡明显受到抑制；（２）耐药细胞株中ｂｃｌ２基因表达明显强于敏感细胞株。结论凋亡抑制基因ｂｃｌ２在人膀胱癌多药耐药细胞中的高表达，是导致其细胞凋亡受抑制的重要原因，对于人膀胱癌多药耐药的产生具有重要意义。

Objective To evaluate the inhibition effect of liposome conjugated antisense oligonucleotides on the BIU 87 proliferation. Methods The human bladder cancer cell line BIU 87 was treated with antisense oligodeoxynucleotides targeting the mRNA encoding proliferating cell nuclear antigen (PCNA) with lipofectin.The inhibition of proliferation was studied with cell growth curves and MTT method.The expression of PCNA was detected immunohistochemically. Results The cell proliferation was inhibited effectively by antisense oligodeoxynucleotides with lipofectin ( P <0.05).The protein expression of PCNA was inhibited completely at 12 hours and effectively at 24 to 36 hours ( P <0.01).Lipofectin could enhance the effect by about 50 times.The sense oligodeoxynucleotides showed no such effect ( P <0.01). Conclusions Antisense oligodeoxynucleotides conjugated with lipofectin might be a hopeful method to inhibit the proliferation of bladder cancer cell by inhibiting the expression of PCNA.Further investigations are needed in order to use this method in bladder cancer therapy.