妊娠高血压综合征与血管紧张素转化酶关系的研究

THE STUDY OF DETECTION OF INSERTION/DELETION POLYMORPHISM OF ANGIOTENSIN CONVERTING ENZYME GENE IN POREECLAMPSIA

为探讨血管紧张素Ⅰ转化酶（ＡＣＥ）基因第１６内含子插入／缺失多态性与妊娠高血压综合征的关系，应用聚合酶链反应技术，检测６０例妊高征患者及３８例正常妊娠妇女ＡＣＥ基因的插入／缺失状态。６０例妊高征患者中，ＡＣＥ基因Ｉ型和ＤＤ型频率分别为１５％（９／６０）和６５％（３９／６０），而３８例正常晚妊妇女中则分别为５０％（１９／３８）和１０．５％（４／３８）。提示Ｉ型基因是妊高征的保护性基因。

To establish a method for sensitive,specific and rapid detection of the genotypes of angiotensin converting enzyme (ACE) gene and to study the relationship between the ACE gene polymorphism and preeclampsia, a pair of primers for the exon 16 of ACE gene was designed, and a sensitive and specific method for detection of the ACE region of the insertion/deletion was determined by polymerase chain reaction(PCR). The subjects were classified,according to the presence or absence of a 287bp insertion in intron 16 of the ACE gene ,as Ⅱ, DD,and heterozygotes for deletion/insertion(DI).The study reveals genotype Ⅱ of ACE is a marker for reduced risk for preeclampsia while DD is a risk gene.