摘要
为探讨血管紧张素Ⅰ转化酶(ACE)基因第16内含子插入/缺失多态性与妊娠高血压综合征的关系,应用聚合酶链反应技术,检测60例妊高征患者及38例正常妊娠妇女ACE基因的插入/缺失状态。60例妊高征患者中,ACE基因I型和DD型频率分别为15%(9/60)和65%(39/60),而38例正常晚妊妇女中则分别为50%(19/38)和10.5%(4/38)。提示I型基因是妊高征的保护性基因。
To establish a method for sensitive,specific and rapid detection of the genotypes of angiotensin converting enzyme (ACE) gene and to study the relationship between the ACE gene polymorphism and preeclampsia, a pair of primers for the exon 16 of ACE gene was designed, and a sensitive and specific method for detection of the ACE region of the insertion/deletion was determined by polymerase chain reaction(PCR). The subjects were classified,according to the presence or absence of a 287bp insertion in intron 16 of the ACE gene ,as Ⅱ, DD,and heterozygotes for deletion/insertion(DI).The study reveals genotype Ⅱ of ACE is a marker for reduced risk for preeclampsia while DD is a risk gene.
出处
《山东医科大学学报》
1999年第1期21-23,共3页
Acta Academiae Medicinae Shandong
关键词
妊娠高血压
综合征
ACE
Pregnancy complications, cardiovascular
Hypertension
Angiotensin converting enzyme