附子膨大充实期干物质及有效成分积累规律研究

Evaluation of the Accumulation of Dry Substance and Active Components of Radix Aconiti Lateralis Praeparata

目的:通过对附子膨大充实期干物质及有效成分积累规律研究,为附子适宜采收期的确定提供依据。方法:定期采样,测定附子的鲜重、干重、折干率;滴定法检测生物碱含量;HPLC法同时测定3种双酯型生物碱新乌头碱、乌头碱、次乌头碱的含量。结果:在膨大充实期内,附子生长分为快速增长期、稳定期、衰减期三个阶段;生物碱的含量在一定范围波动,新乌头碱、乌头碱、次乌头碱的含量增长缓慢;生物碱总量、新乌头碱总量、乌头碱总量、次乌头碱总量在4月下旬至6月中旬随着块根增大呈现快速增长,于6月下旬趋于平稳,7月上旬后因处于高温高湿季节,地下块根逐渐腐烂而下降。结论:6月下旬至7月上旬,江油附子正处于产量与有效成分含量的最高和稳定期,为适宜采收期。

Objective：To study the accumulation rules of dry substance and active components in the Radix Aconiti Lateralis Praeparata for providing the basic evidences to collection time.Methods：The Roots were collected periodically and their fresh weight and dry weight were measured.The contents of total alkaloids and three diester aconitum alkaloids aconitine,hypaconitine and mesaconitine were determined by titration and HPLC respectively.Results： During the stage of root inflation,the content of total alkaloids was at a certain level,while the content of diester aconitum alkaloids were increased gradually.The total amount of alkaloid and diester aconitum alkaloids were increased rapidly from the last third of April to the middle third of June,and then maintained at relative constant levels and were decreased from the first third of July.Conclusion： It is more preferable to collect the Radix Aconiti Lateralis Praeparata during the period from the last third of June to first third of July.