朝鲜白头翁总皂苷提取工艺的优化

Optimization of Extraction Technics of Total Saponins from Pulsatilla cernua

目的：通过单因素和正交试验优化朝鲜白头翁中总皂苷的超声波提取工艺。方法：以白头翁皂苷B4为对照品,采用分光光度法进行含量测定。结果：确定皂苷的最大吸收波长为470 nm,皂苷含量在0-0.040 mg/mL范围内与吸光度呈良好线性关系。最佳提取工艺为：乙醇浓度80%,超声时间40 min,料液比1∶20,超声功率80W,提取1次。其中乙醇浓度对总皂苷提取影响最大,此工艺条件下朝鲜白头翁中总皂苷含量为4.32%。结论：该方法提取效率高、稳定、准确、重现性好。

Objective： The extraction condition of total saponins from Pulsatilla cenua by ultrasonic wave was optimized by single factor and orthogonal experiments.Methods： The largest absorbency of saponin was intended to be 470 nm by wavelength scan method with the pulchinenoside B4 as control sample,the linear relationship was observed between the absorbency and the content of saponin in the range of 0-0.040 mg/mL.Results： The optimal conditions of extraction was as following： 80% of alcohol concentration,40 min of ultrasonic time,1∶20 of solid to liquid ratio,80 W of ultrasonic power and one time for extraction.Among them,alcohol had the most significant effect on the extraction of total saponins.Conclusion： The content of total saponins in Pulsatilla cernua was 4.32% under the optimal condition.The method developed here is efficient,stable,accurate and repeatable.