定量免疫PCR检测血清胃肿瘤相关MGAgs新方法的建立及在胃癌早期诊断中的预警价值

A Novel Approach for the Detection of Gastric Carcinoma-associated MGAgs by Serum Quantitative Immuno-PCR and Its Significance in Diagnosis of Early Gastric Cancer

建立定量免疫PCR检测血清胃肿瘤相关MGAgs新方法及研究其在胃癌早期诊断中的预警价值。采用定量免疫PCR技术检测43例胃癌、37例健康献血员血清中MGAgs的含量。研究显示,血清MGAgs含量在健康对照组与胃癌组组间存在显著差异(P<0.01),应用定量免疫PCR方法检测50μL血清MGAgs的截断值相当于为5972个MKN45细胞裂解所得抗原。研究表明,定量免疫PCR技术检测血清MGAgs抗原具有较高的灵敏性和稳定性,通过对胃癌患者血清中MGAgs进行定量检测,可能用于胃癌的诊断和鉴别诊断。

To explore a novel approach for the detection of gastric carcinoma-associated MGAgs by serum quantitative immuno-PCR and to confirm its significance in diagnosis of early gastric cancer,the serum levels of MGAgs were quantifying by real time immuno-PCR in 43 cases of gastric cancer and 37 cases of healthy donor. There was statistical difference （P0.01） in serum MGAgs between gastric cancer and healthy donor by using the real time immuno-PCR. As real time immuno-PCR shown that the cut-off value of MGAgs for gastric cancer was equivalent to 5972 MKN45 cells in 50 μL of serum. The quantitative detection of gastric carcinoma-associated MGAgs in serum from patients with gastric cancer by real time quantitative immuno-PCR is highly sensitive and maybe useful for the diagnosis of gastric cancer.