三氟乙酰伯氨喹及其合成前体伯氨喹的高效毛细管电泳分析

Analysis of Trifluoroacetyl Primaquine and Primaquineby High Performance Capillary Electrophoresis

目的：建立适合抗疟新药三氟乙酰伯氨喹及其合成前体伯氨喹的高效毛细管电泳定量检测方法。方法：在Ｂｉｏ－ＲａｄＨＰＥ－１００型毛细管电泳仪上，以自由区带电泳分离模式探讨电泳体系各因素对伯氨喹及三氟乙酰伯氨喹分离的影响；并选择甲基麻黄素为内标，柱上２１０ｎｍ检测，进行定性定量分析。结果：伯氨喹、三氟乙酰伯氨喹在测定浓度范围内，峰面积比与被测物浓度的线性关系良好。重现性考察结果表明，６次测量迁移时间的相对标准差小于０６％，峰面积比的日内日间相对标准差小于５０％。结论：高效毛细管电泳在抗疟新药三氟乙酰伯氨喹及其合成前体伯氨喹定性定量方面的成功应用，为药物合成过程产品质量控制提供更为简便、快速的检测手段。

Objective: To establish a high performance capillary electrophoresis(HPCE)method for the analysis of anti-malarial new drug trifluoroacetyl primaquine(M8506)and its synthetic material primaquine(PQ ) Methods: Based on the mode of capillary zone electrophoresis (CZE)in the Bio-Rad HPE-100 capillary electrophoresis system,effects of some factors in the electrophoretic conditions on the separation of PQ and M8506 were investigatedAn assay procedure was carried out using methyl ephedrine as internal standard with on-column UV monitoring at 210 nm Results: A good linear relationship was obtained between the ratio of peak area of sample and internal standard and corresponding concentration of sample ,the relative standard deviations of migration time and the ratio of peak area of within-day and between-day for replicate injections(n=6) were not more than 06 % and 50 %,respectively Conclusion: The results demonstrated that HPCE would be a simple and quick qualitative and quantitative met