几种中草药中痕量锗的同步荧光法测定

Determination of Trace Amount of Germanium in Some ChineseHerbal Medicine by Synchronous Fluorimetry

目的：测定中草药中痕量锗。方法：采用同步荧光分析法。在乙醇介质中，以磷酸调节试液酸度，桑色素乙醇溶液为络合剂。结果：Ｇｅ－桑色素络合物的最佳峰位λｅｘ＝４２７ｎｍ，Δλ＝７０ｎｍ。在０～２００ｎｇ·ｍＬ－１浓度范围内线性关系良好（ｒ＝０９９９６），最低检出限为０５ｎｇ·ｍＬ－１。结论：实验证明，该法用于中草药中痕量锗的测定灵敏方便。

Objective: A synchronous fluorimetry method has been developed to determine the chinese herbal medicine. Method: In ethanol medium,phosphoric acid was used to adjust the acidity of the solution,morin-C\-2H\-5OH solution as the complex reagent. Results: The optium wavelength of Ge-Morin complex at ex=427 nm,=70 nm.The linear ranges was 0200 ngmL-1(r=09996).The detection limit was 05 ngmL-1. Conclusion: The method is sensitive and convenient for determination of chinese herbal medicine.It has been applied to the determinations of some marketing samples and the results are satisfactory.