国内外不同厂家色谱柱对人胰岛素注射液分离效果的考察

Suggestion and Evaluation of Chromatographic Columns\=from Different Domestic and Foreign Manufacturers for the\=Separation of Human Insulin Injection by HPLC

目的：比较国内外不同厂家色谱柱对人胰岛素注射液的分离效果。方法：以不同配比的硫酸盐缓冲液和５０％乙腈溶液为流动相，检测波长为２１４ｎｍ。结果与结论：通过调整流动相的不同配比，国内外不同厂家色谱柱均可使人胰岛素与脱酰胺胰岛素及防腐剂得到良好分离。

Objective: We make a comparision of columns between domestic and Vydae for separation of human insulin injection by HPLC. Method: The mobile phase is sodium sulfate buffer(pH 23)-50 % acetonitrile (4555) or (4753).Detection wavelength is 214 nm. Result and Conclusion: It has been shown that columns from different domestic and foreign manufacturers could be successfully applied to separate human insulin and its antiseptic by HPLC after appropriately adjusting the proportion of the same mobile phase.