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Identification and Functional Analysis of Differentially Expressed Genes of Ascaris suum Goeze,1782 from Ascaris lumbricoides Linnaeus, 1758

Identification and Functional Analysis of Differentially Expressed Genes of Ascaris suum Goeze,1782 from Ascaris lumbricoides Linnaeus, 1758
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摘要 In order to provide further evidence to prove that Ascaris suum Goeze,1782 and Ascaris lumbricoides Linnaeus,1758 are really different species in taxonomy,and to identity A.suum larval migrans-related genes for diagnosis and prevention use,A.suum genes that were differentialy expressed from the same gender of A.lumbricoides were enriched by subtracting the same expressed genes using suppression subtractive hybridization （SSH） assay.Specificity of the selectively enriched cDNA was verified by Southern blot analysis.The female A.suum specific cDNA library was then constructed and sequenced.Basic local alignment search tool （BLAST） analysis of female A.suum specific cDNA identified 6 specific ESTs with tentative functions related to larva migrans.This study provided further evidence for differentiating A.suum from A.lumbricoides.Mining for the detailed information and application of the 6 ESTs are worth being done in the future studies. In order to provide further evidence to prove that Ascaris suum Goeze,1782 and Ascaris lumbricoides Linnaeus,1758 are really different species in taxonomy,and to identity A.suum larval migrans-related genes for diagnosis and prevention use,A.suum genes that were differentialy expressed from the same gender of A.lumbricoides were enriched by subtracting the same expressed genes using suppression subtractive hybridization （SSH） assay.Specificity of the selectively enriched cDNA was verified by Southern blot analysis.The female A.suum specific cDNA library was then constructed and sequenced.Basic local alignment search tool （BLAST） analysis of female A.suum specific cDNA identified 6 specific ESTs with tentative functions related to larva migrans.This study provided further evidence for differentiating A.suum from A.lumbricoides.Mining for the detailed information and application of the 6 ESTs are worth being done in the future studies.
作者 WU Shao-qiang LIN Xiang-mei ZHU Xing-quan
机构地区 Institute of Animal and Plant Quarantine State Key Laboratory of Veterinary Etiological Biology
出处 《Agricultural Sciences in China》 CAS CSCD 2010年第6期903-910,共8页 中国农业科学（英文版）
基金 supported by China Postdoctoral Science Fundation (2004035591) the China National Science Funds for Distingusied Young Scientists (30225033)
关键词 Ascaris suum Ascaris lumbricoides NEMATODE suppression subtractive hybridization EST larva migrans Ascaris suum, Ascaris lumbricoides, nematode, suppression subtractive hybridization, EST, larva migrans
分类号 Q78 [生物学—分子生物学] S858.285.9 [农业科学—临床兽医学]
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Agricultural Sciences in China
2010年 第6期

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