玉米(Zea mays L.)中凋亡相关基因c-myc同源序列的检出及其染色体原位杂交定位

DETECTION OF THE SEQUNENCES HOMOLOGOUS TO C-MYC IN MAIZE AND THEIR CHROMOSOME LOCATIONS VIA IN SITU HYBRIDIZATION

原癌基因c-myc是普遍存在于动物组织中的一个高度保守的细胞凋亡相关基因,决定着多种动物细胞的凋亡。我们首次以人c-myc的基因组DNA为探针,用生物素标记的原位杂交和酶联级联放大检测系统在玉米中检出了c-myc基因的同源序列,并对其进行了染色体物理定位。在第5染色体长臂近末端、第4染色体长臂近着丝粒及第1染色体短臂近着丝粒处检测到杂交信号,信号与着丝粒的百分距离分别为96.21±4.46、24.11±0.47和10.02±1.04,本结果为寻找和研究植物细胞凋亡基因提供了重要线索。

The highly conserved proto-oncogene c-myc in animals has been shown to play a central role in regulating multi-ple developmental processes including growth, proliferation, differentiation and apoptosis. Since c-myc is highly conserved, investigation of it in plants is warranted. In this paper, we detected and localized the sequences homologous to c-myc in maize (Zea mays L. ) via chromosome in situ hybridization for the first time. It showed that the signals of the probe corresponding to c-myc from human were detected in the long arms of the fifth and the fourth chromosomes and in the short arm of the first one repeatedly. The average percentage distances from centromere to signal site were 96. 21±4. 46,24. 11±0. 47 and 10. 02±1. 04 respectively. The results provided a valuable insight into searching for genes associated with apoptosis in plants.