人卵黄囊造血的探讨

STUDY ON HUMAN YOLK SAC HEMATOPOIESIS

采用卵黄囊组织切片、涂片的形态学、细胞化学染色、造血干/祖细胞体外培养及CD_(34)单克隆抗体免疫荧光检测等方法研究表明:人卵黄囊中存在造血岛,造血岛内由于造血微环境的特点致使此期造血主要向红系分化。血岛中检测出CD_(34)^+细胞,比例高于胎肝及成人骨髓,干/祖细胞于体外培养形成红系集落。结论:人胚胎期造血源于卵黄囊。

The hematopoiesis of human yolk sac and the biological characteristics of hemopoietic stem/progenitor cells ere invcstigutcd by using morphological analysis of histological sections, smears, culture of hemopoietic stem/progenitor celis in vitro and detecting of CD34+ cells by monoclonal antibodies. It is found that there are many blood islands in human yolk sac. The yolk sac hematopoietic cells are restricted to the erythroid lineage due to characteristics of the hematopoietic microenvironment. The stem/progenitor celis can form erythropoietic colonies in vitro. There are CD34+ cells in blood islands and the percentage is higher than cells from embryonic. liver and adult bone marrow. It is concluded that the human yolk sac is the original site of hematopoiesis.