摘要
以金黄地鼠精母细胞为材料，以抗肌动蛋白抗体为探针，应用免疫荧光和免疫胶体金技术对ＳＣ有无肌动蛋白的问题进行了研究。免疫荧光结果表明：经抗肌动蛋白抗体标记后，减数分裂粗线期标本中ＳＣ发出特异性荧光，说明肌动蛋白存在于ＳＣ中。免疫电镜结果表明：实验组ＳＣ的胶体金颗粒密度远高于对照组的金颗粒密度，说明ＳＣ含有肌动蛋白。观察到，常染色体ＳＣ和性染色体ＳＣ以及偶线期和粗线期ＳＣ中都含有肌动蛋白，肌动蛋白分布于ＳＣ的端部和侧生组分上，代表肌动蛋白的胶体金颗粒在ＳＣ上往往成簇存在。对ＳＣ含有肌动蛋白的意义进行了讨论。
pinions upon whether actin is Fresent in synaptonemal complexes of eukaryotesare still controversial. We detected actin in synaptonemal complexes cf spermatocytesof Mesocricetus auratus with indirect immunofluorescence technique and observedbright fluorescence in the SCs, indicating the Fresence of actin in the structure. Welabelled sections of the spermatocytes of M .auratus with immunogold technique,goldParticels were observed over the lateral elements and attachment plaques of the SCs,and many of the gold were found in clusters. confirming the results of theimmunofliorescence observations. Furtlter examinations revealed the gold particlesrepresenting actin in SCs of autosomes and sex chromcsomes and also in SCs ofzygotene and Pachytene Our results are in favour of the point of view that actin is acomponent of the SC.
基金
国家教委留学归国人员基金
关键词
金黄地鼠
联会复合体
肌动蛋白
免疫荧光
Actin, Immunofluorescence Immunogold technique. Mesocricetus auratus,Synaptonemal complexes
