摘要
应用TISH对小偃麦部分双二倍体TAF46(2n=8x=56)及其衍生的6个二体异附加系的中间偃麦草染色体组种类进行了分析、鉴定。以拟鹅冠草(Ps.strigosa)DNA为探针的分析结果表明,TAF46所含有的中间偃麦草染色体组为合成染色体组,即6条St组染色体和8条E组染色体。在其衍生的二体异附加系中,L4和L7含有St组染色体,L1、L2、L3、L5含有E组染色体。TAF46所含有的中间偃麦草染色体的部分同源群依次为IE(L3)、2St(L6)、3E(L2)、4St(L4)、5E(L5)、6St(L7)、7E(L1)。
ISR technique was applied tc TAF46 a partial amphiploid (2n = 8x = 56)derived from a Thinopyurm intermedium×Triticum aestivum hybrid and to 6 o thepossible disomic addition lines derived from it to determine the genome origin of thealien genome and the addition lines. The probe employed was total genomic DNA ofPseudoroegneria strigosa.It was determined that the alien genome in TAF46 was asynthetic genome containing 6 St genome chromosomes and 8 from tlte E genome. Stgenome chromosomes were detected in two addition lines and E genome chromosomesin the remaining 4 The homoeologous groups of alien chromosomes in TAF46 wereIE (L3), 2St 3E(L2), 4St(L4), 5e(L5),6St(L7), and 7E(L1).
基金
国家自然科学基金
关键词
合成染色体组
小偃麦
异附加系
GISH
Partial amphiploid, Synthcbc genome, Thinopyrum-wheat,Additior line,GISH