小偃麦部分双二倍体及其异附加系异源染色体的GISH分析

GISH Analysis of Genome Origin of Alien Chromosomes in Thinopyroninter medium-Wheat Partial Amphiploids and Derived Addition Lines

应用ＴＩＳＨ对小偃麦部分双二倍体ＴＡＦ４６（２ｎ＝８ｘ＝５６）及其衍生的６个二体异附加系的中间偃麦草染色体组种类进行了分析、鉴定。以拟鹅冠草（Ｐｓ．ｓｔｒｉｇｏｓａ）ＤＮＡ为探针的分析结果表明，ＴＡＦ４６所含有的中间偃麦草染色体组为合成染色体组，即６条Ｓｔ组染色体和８条Ｅ组染色体。在其衍生的二体异附加系中，Ｌ４和Ｌ７含有Ｓｔ组染色体，Ｌ１、Ｌ２、Ｌ３、Ｌ５含有Ｅ组染色体。ＴＡＦ４６所含有的中间偃麦草染色体的部分同源群依次为ＩＥ（Ｌ３）、２Ｓｔ（Ｌ６）、３Ｅ（Ｌ２）、４Ｓｔ（Ｌ４）、５Ｅ（Ｌ５）、６Ｓｔ（Ｌ７）、７Ｅ（Ｌ１）。

ISR technique was applied tc TAF46 a partial amphiploid (2n = 8x = 56)derived from a Thinopyurm intermedium×Triticum aestivum hybrid and to 6 o thepossible disomic addition lines derived from it to determine the genome origin of thealien genome and the addition lines. The probe employed was total genomic DNA ofPseudoroegneria strigosa.It was determined that the alien genome in TAF46 was asynthetic genome containing 6 St genome chromosomes and 8 from tlte E genome. Stgenome chromosomes were detected in two addition lines and E genome chromosomesin the remaining 4 The homoeologous groups of alien chromosomes in TAF46 wereIE (L3), 2St 3E(L2), 4St(L4), 5e(L5),6St(L7), and 7E(L1).