爱德华氏菌产乳酸氧化酶的条件研究

STUDIES ON THE LACTATE OXIDASE PRODUCING CONDITIONS BY EDWARDSIELLA TARDA

在以前工作的基础上，对已获得的产乳酸氧化酶的５株菌进行复筛，对产酶量大的一株菌进行了分类鉴定，确定该菌株属迟钝爱德华氏菌生物群Ｉ（ＥｄｗａｒｄｓｉｅｌａｔａｒｄａＢｉｏｇｒｏｕｐＩ）。这与曾报道的产乳酸氧化酶分枝杆菌（Ｍｙｃｏｂａｃｔｅｒｉｕｍ）和片球菌（Ｐｅｄｉｏｃｏｃｕｓ）是不同的菌。分别研究了培养基中的培养初始ｐＨ、核黄素、乳酸钠以及硫酸铵对发酵产乳酸氧化酶的影响。这一酶源在酶法生产丙酮酸及医疗诊断和酶电极应用上有意义。

A bacterium producing lactate oxidase was re screened from five strains based on previous researches.The lactate oxidase activity was the highest in strain L1 and this stain was chosen as the enzyme source.Morphological and physiological studies revealed that the bacterial strain L1 belongs to the Edwardsiella tarda Biogroup I.This stain is different from the reported strains Mycobacterium and Pediococcus, which produce lactate oxidase.The enzyme producing conditions were studied in shaking cultures,and the effects of initial pH,riboflavin,lactate and ammonia sulphate concentrations on the production were carried out respectively.The bacteria resource of enzyme is significant to pyruvate production by enzymatic method,and to the enzyme assay of lactate for medical diagnosis,and the application of enzyme electronic probe.