土壤来源的五个苏云金芽孢杆菌新亚种的鉴定

THE IDENTIFICATION OF 5 NEW SEROTYPES OF BACILLUS THURINGIENSIS FROM SOIL IN CHINA

从中国土壤中分离的大量苏云金芽孢杆菌菌株中鉴定出Ｈ４２、Ｈ４３、Ｈ５６、Ｈ６０及Ｈ６２等５种新Ｈ血清型，并进行了形态、培养特征、生化反应、晶体蛋白质成分及毒力特性等项检测鉴定，鉴定了５个苏云金芽孢杆菌新亚种：Ｂａｃｉｌｕｓｔｈｕｒｉｎｇｉｅｎｓｉｓｓｕｂｓｐ．ｊｉｎｇｈｏｎｇｉｅｎｓｉｓ（Ｈ４２），Ｂ．ｔｈｕｒｉｎｇｉｅｎｓｉｓｓｕｂｓｐ．ｇｕｉｙａｎｇｉｅｎｓｉｓ（Ｈ４３），Ｂ．ｔｈｕｒｉｎｇｉｅｎｓｉｓｓｕｂｓｐ．ｒｏｎｇｓｅｎｉ（Ｈ５６），Ｂ．ｔｈｕｒｉｎｇｉｅｎｓｉｓｓｕｂｓｐ．ｐｉｎｇｌｕｏｎｓｉｓ（Ｈ６０）及Ｂ．ｔｈｕｒｉｎｇｉｅｎｓｉｓｓｕｂｓｐ．ｚｈａｏｄｏｎｇｅｎｓｉｓ（Ｈ６２）。毒力生物测定证明５个新亚种的代表菌株对棉铃虫（Ｈｅｌｉｏｔｈｉｓａｒｍｉｇｅｒａ）、小菜蛾（Ｐｌｕｔｅｌａｘｙｌｏｓｔｅｌａ）、柳蓝叶甲（Ｐｌａｇｉｏｄｅｒａｖｅｒｓｉｃｏｌｏｒａ）幼虫均无毒力。Ｈ４２、Ｈ４３、Ｈ５６、Ｈ６０对埃及伊蚊（Ａｅｄｅｓａｅｇｙｐｔｉ）、斑须按蚊（Ａｎｏｐｈｅｌｅｓｓｔｅｐｈｅｎｓｉ）及尖音库蚊（Ｃｕｌｅｘｐｉｐｉｅｎｓ）亦均无毒；Ｈ６２对埃及伊蚊无毒，但对尖音库蚊与斑须按蚊有低毒。

Five new strains of Bacillus thuringiensis (Bt) from soil in China were classified. 5 new H serotypes:H42,H43,H56,H60 and H62 were identified. There were some distinction in the morphology, biochemical reaction and toxicity against species of insect. Five new Bt subspecies were identified as follows: Strain YGd22 03 Bacillus thuringiensis subsp. jinghongiensis, H42;Strain KK31 01 Bacillus thuringiensis subsp. guiyangiensis ,H43;Strain SCg04 02 B.thuringiensis subsp. rongseni ,H56;Strain NXP15 04 B.thuringiensis subsp. pingluonsis ,H60;Strain HZ39 04 B.thuringiensis subsp. zhaodongensis ,H62.