摘要
目的了解天津市腹泻婴幼儿腺病毒的感染情况及其分型特点。方法于2008年4月—2009年4月收集天津市儿童医院住院部病毒性腹泻患儿粪便标本766例,通过PCR法确定阳性标本,将阳性标本进行细胞培养产生细胞病变后,再次PCR扩增确证,并将产物测序,进行基因型别分析。结果 766例标本中腺病毒的检出率为17.62%,其中肠道腺病毒Ad41型占阳性标本的86.67%,Ad1型占13.33%,Ad40型未检出。感染腺病毒患儿的年龄中位数为8.00月龄(0.06~24月龄),全部分布在2岁以下。腺病毒感染全年存在,尤以7月感染最多,其次是2月和9月。结论肠道腺病毒是天津市住院婴幼儿病毒性腹泻的主要病原体之一,2008年4月—2009年4月以Ad41型流行为主。
Objective To determine the prevalence and the molecular characterization of enteric adenovirus infection among diarrheic infants and children in Tianjin. Methods A total of 766 stool samples collected from infants and children with viral diarrhea hospitaled at the Tianjin Children's Hospital during April,2008-April,2009 were examined for the presence of adenoviruses by PCR first,then the adenovirus-positive samples were cultured by cell culture. The CPE-samples were examined again by PCR,the positives were subjected to molecular genetic analysis by sequencing method. Results Adenoviruses were detected in 135 samples (135/766, 17.62%).The most frequent type identified was Ad41 occurring in 86.67% of the cases, then followed by Adl (13.33%) .There was no Ad40 serotype found. Among the adenovirus-positive samples, the median (range) age of the children was 8.00 months old (0.06-24 months old), and the detection of adenoviruses was all in children under 2 years old. Adenoviruses could be identified throughout the year with the peaks in July, February, and September. Conclusion Enteric adenovirus is one of the major pathogens of viral diarrhea among hospitalized children with diarrhea in Tianjin and Ad41 is a predominant genotype during April,2008-April,2009.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2010年第6期504-506,F0003,共4页
Journal of Environment and Health
基金
国家自然科学基金重点项目(30930078)
关键词
腺病毒
腹泻
聚合酶链反应
基因型
Adenovirus
Diarrhea
Polmerase chain reaction
Genotype