饮料中乙酰磺胺酸钾的离子色谱测定法

Determination of Acesulfame Potassium in Beverages by Ion Chromatography

目的建立饮料中乙酰磺胺酸钾的离子色谱-电导检测器测定方法。方法采用IonPac誖AS11-HC为分离柱,IonPac誖AG11-HC为保护柱,25mmol/L氢氧化钾作为淋洗液,柱流量1.0ml/min,电导检测器检测,采用色谱保留时间定性,峰面积定量进行测定。结果该方法的线性范围为0.2～5.0μg/ml,R2=0.9992,RSD为1.53%～2.66%,回收率为98.0%～105%,检出限为0.002μg/ml。结论该方法简单、快速、准确,适用于饮料中乙酰磺胺酸钾含量的测定。

Objective To establish a method for the determination of acesulfame potassium in beverages by ion chromatography. Methods The ion chromatography separation was conducted by using an IonPac AS11-HC （4 mm×250 mm） analytical column, an IonPac AG11-HC （4 min×50 mm） guard column, and an ED50 electrochemical detector. The acesulfame potassium was quantified by measuring peak area. 25 mmol/L potassium hydroxide was used as the eluent with flow rate of 1.0 ml/min. Results The linearity of the method ranged from 0.2 to 5.0 μg/ml （R2=0.999 2）, the RSDs were between 1.53% and 2.66%, The recovery rates were between 98.0% and 105%, and the limit of detection was 0.002 μg/ml. Conclusion This simple and accurate method is applicable to the determination of acesulfame potassium in beverages.