云南会泽铅锌矿渣中节杆菌的生理特征和系统发育

Phylogenetic and Physiological Characteristics of Three Arthrobacter spp.from Huize Lead-zinc Mine Tailings in Yunnan Province

对分离自铅锌矿渣中一种具有奶油色不扩散菌落形态的优势细菌进行了系统发育和部分生理特征研究。在可培养的细菌类型中,该种菌落的出现频率在50%左右。利用60个菌株16SrRNA基因约500bp的序列和其相关种构建的系统树表明,这些菌落属于节杆菌属内多个系统发育地位有差异的类群。各类群代表菌株BS11、BS20和AS19约1440bp左右的16SrRNA基因序列分析的结果表明,BS11菌株同Arthrobacter histidinolovorans和Arthrobacter nicotinovorans关系密切,BS20菌株同Arthrobacter chlorophenolicus关系密切,而AS19同Arthrobacter aurescens和Arthrobacter ilicis关系密切。在考察的39个碳源中,3个代表菌株都能利用其中的15个,不能利用其中的12个,而对另外12个的利用存在差异。此外,它们对Zn2+、Pb2+、Cd2+、Cu2+和Co2+等5种重金属的抗性水平都较高,但对链霉素、氨苄青霉素、卡那霉素和利福平4种抗生素较敏感。它们是潜在的新物种。

Some numerically dominant colonies,with creamy and nondiffusionable morphology,were isolated from lead-zinc tailings and their phylogenetic and physiological characteristics were determined.This type of colony represented approximate 50% of cultivable bacteria of tailings.Phylogenetic tree constructed from about 500 bp of 16S rRNA gene of 60 isolates and their relatives indicated that,these strains belonged to the member of Arthrobacter genus and were divided into several phylogenetically distinct groups.Three representatives from each group,BS11,BS20 and AS19,respectively,were sequenced approximate 1440 bp of 16S rRNA gene and their phylogeneic positions were further determined.Strain BS11 was phylogenetically close to A.histidinolovorans and A.nicotinovorans,BS20 close to A.chlorophenolicus,and AS19,to A.aurescens and A.ilicis.Among 39 carbon sources tested,three strains could strongly utilize 15 and weakly utilize 12 carbon sources.All of three strains showed high resistance to Pb（NO3）2,ZnSO4,CdCl2,CuSO4 and CoCl2,but relatively sensitive to ampicilin,streptomycin,kanamycin and rifimpicin.Our data suggested these strains may be potential novel species.