柞蚕蛹期灵菌败血病Serratia marcescens C3菌株分离鉴定

Isolation and Identification of Serratia marcescens C3:The Pathogen Causing an Antheraea pernyi Pupal Bacterial Disease

以柞蚕(Antheraea pernyi)蛹为替代寄主繁殖白蛾周氏啮小蜂(Chouioia cunea)技术在辽宁、北京、天津、上海、河北、山东等地美国白蛾(Hyphantria cunea)的生物防治中发挥了重要作用。利用柞蚕蛹繁殖白蛾周氏啮小蜂时,柞蚕蛹期软化病是繁蜂的主要障碍。通过对利用柞蚕蛹繁蜂时蛹内组织液化后呈粉红色这一未知软化病的典型症状进行病原细菌的分离和纯化,得到C3菌株。经Biolog系统和16S rRNA序列分析,鉴定C3菌株为灵菌(Serratia marcescens),经过柯赫法则检验,确定灵菌C3菌株是导致柞蚕蛹期灵菌败血病的病原菌。描述了繁蜂时柞蚕蛹期灵菌败血病发病期的认别特征。

The technique of using the pupae of Chinese tussah,Antheraea pernyi,as a surrogate host to artificially rear Chouioia cunea has been playing an important role in the biological control of the fall webworm,Hyphantria cunea,in Liaoning,Beijing,Tianjin,Shanghai,Hebei,Shandong Provinces or Municipalities and other places of China.Bacterial diseases of Chinese tussah pupae are the main obstacle to mass rearing C.cunea.Based on the fact that the tissue in some pupae turned pink after liquefying during rearing C.cunea,which is a typical symptom of an unknown bacterial disease,a bacterium was isolated and purified,and C3 was obtained.C3 was identified as a strain of Serratia marcescens by conventional techniques,Biolog system and 16S rRNA analysis,and was determined as the pathogenic bacterium causing the disease to Chinese tussah pupae by a test based on Koch＇s postulates.The identification characteristics were described of the disease in its incidence in the process of rearing C.cunea.