环介导恒温扩增对莱姆病病原伯氏疏螺旋体的分型鉴定

A Genotype Loop-mediated Isothermal Amplification (LAMP) for Borrelia Burgdorferi of Lyme Disease

使用环介导恒温扩增技术,基于莱姆病病原伯氏疏螺旋体的外膜蛋白A(OspA)基因,针对伯氏疏螺旋体不同的基因型设计特异性引物,对国内主要的莱姆病病原伯氏疏螺旋体的3个基因型进行分型鉴定。研究结果表明,设计的引物具有良好的特异性,可以对狭义伯氏疏螺旋体(Borrelia burgdorferi sensu strict)、嘎氏疏螺旋体(B.afzelii)和伽氏疏螺旋体(B.garinii)进行分型鉴定。伯氏疏螺旋体的分型鉴定可以对不同临床症状莱姆病患者的治疗和莱姆病的控制提供一定的依据。

Borrelia Burgdorferi is the causative pathogens for Lyme disease.A loop-mediated isothermal amplification（LAMP） rapid assay was developed for genotyping Borrelia borgdorferi based on the outer surface protein A（OspA） gene.All three different genotypic isolates including B.burgdorferi sensu stricto,B.afzelii and B.garinii,distributed mainly in China were detected and genotyped successfully using the LAMP method.The genotyping of Borrelia burgdorferi would provide evidence for treatment of patients with different clinical symptoms and for control of Lyme disease.