丝胶对2型糖尿病大鼠睾丸生殖细胞增殖及相关因素的作用

Effects of Sericine on Proliferation and Related Factors of Germ Cells in Testis of Type 2 Diabetic Rats

目的通过观察睾丸增殖细胞核抗原(PCNA)和原癌基因c-fos表达的变化,探讨丝胶对2型糖尿病大鼠睾丸生殖细胞增殖及相关因素的影响。方法 40只雄性SD大鼠随机分成4组(每组n=10),正常对照组、糖尿病模型组、丝胶治疗组和阳性对照组。链脲佐菌素连续腹腔注射建立2型糖尿病大鼠模型;待模型成功建立后,丝胶治疗组和阳性对照组大鼠分别给予丝胶(2.4g·kg-·1d-1)和二甲双胍(55.33mg·kg-·1d-1)灌胃35d。采用ELISA方法检测大鼠血清睾酮水平;采用RT-PCR法和S-P免疫组化法分别检测睾丸PCNA和c-fos的表达。结果糖尿病模型大鼠血清睾酮水平、睾丸PCNA和c-fos的表达均明显低于正常对照组大鼠(P<0.01)。丝胶治疗组和阳性对照组大鼠血清睾酮水平、睾丸PCNA和c-fos的表达明显高于模型大鼠(P<0.01,P<0.05),且两组间比较无明显差别(P>0.05)。结论丝胶可通过上调睾丸PCNA和c-fos的表达,改善糖尿病大鼠的生精功能和雄性激素分泌水平,发挥对糖尿病生殖功能损伤的保护作用。

Objective To investigate the effects of sericine on proliferation and related factors of germ cells in testis of type 2 diabetic rats by studying the changes of proliferating cell nuclear antigen （PCNA）and proto-oncogene c-fos expression in testis. Methods Forty male SD rats were randomly divided into 4 groups（n =10）：normal control group,diabees model group,diabetic group with sericine treatment,and positive control group. The diabetes model was made by continuous intraperitoneal injection of Streptozotocin into rats. After the diabetes animal model was successfully established,the rats in sericine treatment and positive control groups were respectively lavaged with sericine （2.4g·kg-1·d-1）and metformin （55.33 mg·kg-1·d-1）for 35 days. ELISA was used to detect the serum testosterone level. RT-PCR and SP immunostaining were employed to examine the expression of PCNA and c-fos in testis. Results The serum testosterone level,the PCNA and c-fos expression in testis of diabetic rats were obviously lower than normal control rats （P 0.01）. These indicators were significantly higher in sericine treatment group and positive control group than diabetes model rats （P 0.01,P 0.05）. However,there was no obvious differences between sericine treatment and positive control group （P 0.05）. Conclusion Sericine can improve the spermatogenic functions and male hormone secretion by up-regulating PCNA and c-fos expression in testis so as to have protective effects on diabetes reproductive disfunction.