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鸡IGF-Ⅰ基因5′非翻译区TrulⅠ、TaiⅠ多态位点的发现 被引量：2

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摘要 研究采用测序和PCR-RFLP技术对京海黄鸡、AA鸡和尤溪麻鸡IGF-Ⅰ基因5′非翻译区(EF488284)进行多态性检测。结果表明:在5′非翻译区共发生2个SNP突变位点。43bp位置发生A→G的突变导致TurlⅠ酶切位点发生改变,67bp位置发生A→G的突变导致TaiⅠ酶切位点发生改变。PCR-RFLP结果显示2个突变位点完全连锁,产生AA/CC、AB/CD和BB/DD3种基因型。等位基因和基因型在3个品种中分布不一致,AA鸡为纯合型(AA/CC),在京海黄鸡和尤溪麻鸡中出现3种基因型,可以作为新的标记位点进一步研究。
作者 王慧华 王金玉 李源 侯启瑞 张跟喜 施会强
机构地区 扬州大学动物科学与技术学院 江苏京海禽业集团有限公司
出处 《中国畜牧杂志》 CAS 北大核心 2010年第11期13-15,共3页 Chinese Journal of Animal Science
基金 国家肉鸡产业技术体系资助(nycytx-42-G1-05)
关键词 IGF-Ⅰ PCR-RFLP 多态性 鸡
分类号 S831.2 [农业科学—畜牧学]
  • 相关文献

参考文献6

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二级参考文献27

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共引文献19

同被引文献21

  • 1李伟,彭夏雨,孙凤霞,李大全,廖和荣,李应生.玫瑰冠鸡IGF-I基因多态性与体重及屠体性状关系的研究[J].现代农业科技,2007(15):160-162. 被引量：6
  • 2万秋蓓,王志跃,杨海明,郭春燕,赖毓妍.新扬州鸡IGF-1基因5′调控区PstI位点多态性与繁殖性状关系的研究[J].中国畜牧杂志,2007,43(21):1-4. 被引量：7
  • 3Amills M,Jimemea N, Villalba D, et al. Identification of three single nucleotide polymorphisms in the chicken insulin-like growth factor 1 and 2 genes and their associations with growth and feeding traits [J].Poultry Science, 2003, 82(10): 1486-1493.
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中国畜牧杂志
2010年 第11期

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