HPLC-UV-ELSD联用检测熊香胶囊中牛磺熊去氧胆酸的含量

Determination of TUDCA in Xiongxiang Capsule by HPLC - UV - ELSD

目的:HPLC-UV-ELSD联用检测熊香胶囊中牛磺熊去氧胆酸的含量;方法:采用Kromasil C18色谱柱(5μm,4.6mm×250mm),以甲醇-水(0.1%甲酸)(60∶40)为流动相;紫外检测波长为210nm;ELSD漂移管温度105℃,雾化气体(空气)流速2.7L·min-1;结果:牛磺熊去氧胆酸在1.44～5.76μg线形关系良好,紫外检测方式回收率为97%(n=5),RSD2.3%,UV检测方式回收率为101%(n=5),RSD1.5%;结论:与HPLC/UV相比,HPLC/ELSD测定熊香胶囊中牛磺熊去氧胆酸成分的含量结果更准确。

Objective： To establish a method for the determination of TUDCA in Xiongxiang capsule by HPLC-UV-ELSD.Methods： A HPLC methods detected by UV and ELSD has been developed： Kromasil C18 column （ 250mm × 4.6mm,5μm） with mobile phase of methane and 0.1% formic acid;the detection wavelength was 210 nm;The temperature of the drift tube was set at 105℃,the flow rate of gas at 2.7L·min -1.Results： TUDCA in Xiongxiang Capsule could be determined by HPLC-UV and HPLC-ELSD.The recovery was 97% （ n = 5,RSD = 2.3% ） for HPLC/UV,101% （ n = 5,RSD = 1.5% ） for HPLC/ELSD,respectively.Conclusion： Comparing with HPLC/UV,The HPLC/ELSD method was more accurate and specific to determine the content of TUDCA in Xiongxiang capsule.