醒脑开窍法人中穴针刺时间和针刺频率的正交优化研究

Study on the Optimal Stimulating Frequency and Time at"RenZhong"acupoint in"XinNao KaiQiao"Method with the Orthogonal Design

目的:筛选"醒脑开窍"法针刺人中穴改善大脑中动脉阻塞模型(MCAo)Wistar大鼠神经功能缺损评分的针刺频率和时间最优方案。方法:参照Zea-Longa线拴法复制Wistar大鼠MCAo模型,按正交设计分九组并分别施以频率60、180、120次/min,时间180、60、5s不同组合的针刺干预,以Zausinger六分法评分作为神经功能评价效应指标。结果:人中穴的针刺时间和频率均是改善MCAo模型Wistar大鼠神经功能缺损评分显著因素(P<0.05),针刺频率与时间的最优搭配是180次/min,5s。结论:人中穴针刺时间和频率均是改善MCAo模型大鼠神经功能缺损评分显著因素,且针刺频率和时间存在最优搭配方案。

Objective： To select the optimal stimulating frequency and time at＂RenZhong＂acupoint in＂Xinao Kaiqiao＂method to improve the neurological defict score following the middle cerebral artery occlusion （ MCAo） in Wistar rats.Methods： Male Wistar rats subjected to MCAo with suture method by Zea Longa were divided into nine groups according to orthogonal design method and were treated by acupuncture with different match between stimulating frequency and time （ 60 times per minute,180 times per minute,120 btimes per minute;180 seconds,60 seconds,5 seconds,respectively） .At last,neurological function was assessed with six score method by Zuasinger.Results： The stimulating time and frequency at＂RenZhong＂acupoint both are the main factors to improve Wistar rats after MCAo（ P 0.05） ,and the optimal stimulating frequency and time were 180 beats per minute,5 seconds.Conclusion： The stimulating time and frequency at＂RenZhong＂acupoint both are the main factors to improve Wistar rats following MCAo,furthermore,the optimal match between stimulating frequency and time was being objectively.