鸡蛋清中溶菌酶的提取与抑菌作用

The extraction and antibacterial effect of lysozyme from hen-egg white

研究从鸡蛋清中提取与精制溶菌酶的方法,观察溶菌酶对大肠杆菌与金黄色葡萄球菌的抑制作用。从鸡蛋清提取溶菌酶的方法:取出鸡蛋清,过滤,用蒸馏水溶解,加氯化钠,调节溶液pH至5.0,加入溶菌酶晶种,4℃下静置结晶,过滤,可以得到溶菌酶粗品。将粗品溶菌酶溶解,按上述步骤溶解后结晶,重复3次,可以精制溶菌酶。用滤纸片法观察溶菌酶对大肠杆菌与金黄色葡萄球菌的抑菌作用。结果表明,鸡蛋清溶菌酶提取的较优条件是:酶液pH 10.0、氯化钠加入量5%、蛋清液用2倍体积蒸馏水稀释。70℃下保温5min,鸡蛋清溶菌酶的精制率最高。鸡蛋清溶菌酶对大肠杆菌与金黄色葡萄球菌有明显抑制作用,80℃加热30min对鸡蛋清溶菌酶的抑菌作用影响很小。

The extraction and antibacterial effect of Lysozyme from Hen-egg White（HEW）were studied.The extraction process of lysozyme from HEW were as below：HEW were got and filtrated,dissolved with distilled water,NaCl was added,the pH of the solution was adjusted to 5.0,then lysozyme seed was added,the crystalline was accomplished at 4℃,and crude Lysozyme was got.The crude lysozyme was refined by dissolution and recrystallization.The antibacterial effect of lysozyme on Escherichia coli and Staphylococcus aureus was estimated by filter paper method.The results showed that the better processing parameter for HEW extraction were below：the pH of HEW solution was 10.0,HEW was diluted by 2 times volume distilled water and 5%（w/v NaCl HEW）was added.The yield of refining was highest when tempetature kept 5 min at 70℃.HEW had evident antibacterial effect on Escherichia coli and Staphylococcus aureus.Processing at 80℃ for 30 min had nearly no effect on antibacterial effect of HEW.