犬型钩端螺旋体外膜蛋白OmpL1基因的克隆与序列分析

Cloning and Analysis of the Sequence of Outer Membrance Protein Gene of a Serovar Leptospire canicola Interrogans

作者以犬型钩端螺旋体菌株的DNA为模板,采用PCR方法扩增目的基因OmpL1,克隆到pVAX1载体上,并对OmpL1基因测序后进行序列分析。结果获得960bp片段,DNA序列分析结果表明,该菌株的OmpL1基因与报道的七日热型、秋季型、波摩那型、澳洲型钩体的OmpL1基因核苷酸序列相似性分别为99.38%、93.77%、99.58%、99.38%,氨基酸序列相似性分别为100%、95%、100%、100%。证明OmpL1为致病性钩体所具有的保守性抗原成分,可在钩体病的预防和诊断中发挥重要作用。

In this study,the OmpL1 was amplified by PCR from a serovar Leptospire canicola interrogans,and it was cloned into plasmid pVAX1,then sequence analysis was done.These all got an expectation about the 960 bp of special tape,it＇s sequence analysis and gene comparision with Leptospire hebdomadis,Leptospire autumnalis,Leptospire pomona and Leptospire australis,the homology of nucleotide sequences was 99.38%,93.77%,99.58% and 99.38%,the homology of deduced amino acid was 100%,95%,100% and 100%.It indicated that OmpL1 was a conserved antigen among the pathogenic Leptospira species,fusion protein OmpL1 might play an important role in diagnosis and prevention of Loptosirosis.