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济南市手足口病患者肠道病毒71型的分离与鉴定 被引量：5

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摘要 目的从13例2008年、2009年济南市HFMD患者的粪便标本中分离获得EV71病毒并对其基因型进行鉴定。方法按照中国疾病控制预防中心《手足口病标本采集及检测技术方案》的规定对标本中的病毒进行分离和鉴定，对VP1编码基因的部分核苷酸序列进行测序分析。结果分离到6株EV71病毒，均与EV71C4亚型处于同一进化树分支上，与c4亚型的核酸同源率均大于96％。结论济南地区流行的EV71主要为c4亚型，与2008年中国流行的C4亚型EV71相比并未出现明显突变，引起重症的毒株与未引起重症的毒株相比，在VPI蛋白部分片段上没有发现明显的氨基酸差异。
作者 赵元昊 李志会 李鹏 宋楠楠 岳盈盈 纪璇 孟红
机构地区 山东省医学科学院基础医学研究所
出处 《国际病毒学杂志》 2010年第3期92-96,共5页 International Journal of Virology
关键词 肠道病毒71型 基因型 分离鉴定
分类号 R72 [医药卫生—儿科] R4 [医药卫生—临床医学]
  • 相关文献

参考文献14

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二级参考文献15

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共引文献110

同被引文献40

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引证文献5

二级引证文献25

国际病毒学杂志
2010年 第3期

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