TNF-α、IL-1β在腰椎间盘突出症中致痛作用的研究

The role of TNF-α、IL-1β in the sciatica pain of lumbar intervertebral disc herniation

目的探讨肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(inter-leukin-1β,IL-1β)在腰椎间盘突出症根性神经痛中的病理生理机制。方法 23例突出腰椎间盘组织取自因有持续神经根疼痛而行手术的患者。切取的突出腰椎间盘组织用含有TNF-α、IL-1β的1640培养液和单纯培养液分别进行体外孵育72h后,用酶联免疫吸附检测法(enzymlinked immunosorbent assay,ELISA)检测培养液中前列腺素E(2prostaglandin E2,PGE2)的含量。结果加用TNF-α、IL-1β后PGE2的含量明显增加(P<0.05)。结论 TNF-α、IL-1β可能通过刺激突出腰椎间盘组织中PGE2的释放,在根性神经痛中发挥作用。

Objective To study the pathophysiologic mechanisms of tumor necrosis factor-α （TNF-α）、interleukin-1β （IL-1β） in the sciatica pain of lumbar intervertebral disc herniation.Methods Twenty-three herniated lumbar disc tissues were obtained from twenty-three patients undergoing surgery for persistent radiculopathy.The lumbar tissue were incubated in 1640 medium in vitro.After 72 hours of incubation with or without TNF-α and IL-1β,the content of PGE2 in incubation medium were measured with enzymlinked immunosorbent assay （ELISA）.Results The concentration of PGE2 increased markedly with the addition of TNF-α and IL-1β（P0.05）.Conclusion TNF-αand IL-1βmay be involved in the pathophysiology of sciatica pain through stimulating the production of PGE2.