γ射线照射致小鼠胸腺组织信号转导差异基因的变化及意义

Changes in differentially expressed genes related to signaling transduction in thymus of mice after γ-irradiation and their significance

目的探讨6Gyγ射线照射后不同时间小鼠胸腺组织信号转导差异基因表达谱的动态变化。方法雄性C57BL/6小鼠48只,5～6周龄,经6Gyγ射线照射后,分别于照射后1、6、14、28d分批活杀取胸腺。提取胸腺组织总RNA,应用快速高通量cD-NA基因芯片技术检测γ射线照射后的差异表达基因,特别是与信号转导相关的差异基因的表达。结果小鼠经6Gyγ射线照射后,随时间推移,胸腺组织信号转导相关差异表达基因数目逐渐减少,照射后1、6、14、28d差异基因数量分别为126、43、27和0个。辐射所诱导的胸腺组织信号转导差异表达基因在照射后不同病理阶段,涉及不同的信号通路,主要包括Wnt受体信号通路、MAPK信号通路、G蛋白偶联受体信号PI-3K/AKT通路、NIK-I-κB/NF-κB级联信号通路等。结论 6Gyγ射线照射小鼠胸腺组织信号转导基因涉及多个信号通路,并且显示出时间上的明显差异。

Objective To study the dynamic changes in differentially expressed genes correlated with signal transduction in the thymus of mice after 6Gy γ-irradiation. Methods Forty eight male C57BL/6 mice were randomly divided into irradiation group and control group （24 each）. Mice in irradiation group were sacrificed on 1,6,14 and 28d after 6Gy γ-irradiation to obtain the thymus （6 at each time point）. Thymus RNA was extracted. The expression of differentially expressed genes,especially the genes related to signal transduction,was analyzed using high speed and high-flux cDNA gene chip technique. Results With elapse of time after radiation,the number of differentially expressed genes related to signal transduction decreased gradually. For instance,the numbers of differentially expressed genes were 126,43,27 and 0 on 1,6,14 and 28d after irradiation,respectively. Radiation-induced differentially expressed genes related with signal transduction were involved in different pathways at different pathological stages after irradiation. The differentially expressed genes were mainly involved in Wnt receptor signal pathway,mitogen activated protein kinase pathway,G-protein coupled receptor protein pathway,NIK-I-kappa B/NF-kappa B cascade pathway and so on. Conclusions After 6Gy γ-irradiation,differentially expressed genes related to signal transduction in mouse thymus were involved in various pathways,and displayed an obvious difference with elapse of time.