首次从我国大绒鼠中检测到类似Tula样汉坦病毒

First detection of Tula-like Hantanvirus from Eothenomys milelus in China

目的了解云南省鼠类携带汉坦病毒情况及其分子特征。方法在居民区和野外捕鼠,用直接免疫荧光试验检测鼠肺中汉坦病毒抗原,阳性鼠肺用RT-PCR法扩增汉坦病毒汉滩型和汉城型的S基因片段,并进行核苷酸序列测定和分析。结果 2006年9-10月在云南省泸西县共捕获鼠类4属6种267只,其中居民区146只,优势鼠种为黄胸鼠;野外121只,优势种为大绒鼠。居民区鼠类汉坦病毒带病毒率为0.85%(1/117),阳性鼠为黄胸鼠;野外鼠类汉坦病毒带病毒率为6.67%(6/90),阳性鼠为大绒鼠和高山姬鼠。对7份阳性鼠肺作RT-PCR扩增,结果从来自大绒鼠的LX378标本中检测到汉滩型病毒S片段核酸,其序列分析结果显示与Tula汉坦病毒Koziky/5276Ma/94株(AJ223601)核苷酸和氨基酸同源性最高,分别为81%、89.8%,而与汉滩型76-118株、汉城型L99株的核苷酸(氨基酸)同源性分别为73.9%(80.9%)、74.4%(77.9%)。结论首次从我国大绒鼠中检测到类似Tula样汉坦病毒的核酸序列,有关该类汉坦病毒在云南的分布、宿主动物和致病性以及全基因序列特征尚需进一步研究。

In order to understand the distribution and molecular character of hantanvirus in Yunnan province,rodents were collected in residential area and mountainous area of Luxi county from September to October,2006 and rats lungs were detected by direct immunofluorescence assay. Partial S segment was amplified respectively with hantanvirus genotype-specific primers,sequenced and analyzed the homology with other known sequences. A total of 267 rodents from 4 genera and 6 species were collected from Luxi County of Yunnan Province,among which 146 from residential area （the dominant species is Rattus flavipectus） and 121 from mountain area （the dominant species is Eothenomys miletus）. The positive ratio of hantavirus is 0.85%（1/117） among populated area rodents and 6.67%（6/90） among mountain area rodents,which the rodent species of positive reaction to hantavirus antigen in populated area is Rattus flavipetcus,and in mountain is Eothenomys milelusand Apodemus chevieri.LX378 was amplified by hantavirus S segment primers from 7 positive rats lungs which have been detected by immunofluorescent assay. LX378（GenBank No.HM030880）and Tula virus（AJ223601） showed higher nucleotide and amino acids homology,reached up to 81% and 89.8% respectively,while nucleotide （amino acids） homology of LX378 compared with HTN 76-118 strain and SEO L99 strain was only up to 73.9% （80.9%） and 74.4%（77.9%）,respectively. Tula-like hantavirus nucleotide sequence was firstly detected from Eothenomys milelusin China. We need further study on the distribution,the natural reservoir and the pathogenicity of of Tula-like hantavirus virus in Yunnan province.