HLA-DRB1基因多态性与新疆维吾尔族人群结核病易感性的相关性研究

Correlation between the polymorphism of HLA-DRB1 gene and the susceptibility to pulmonary tuberculosis in population of Uygur Nationality in Xinjiang

目的探讨人类白细胞抗原(HLA)-DRB1基因多态性与新疆维吾尔族人群结核病易感性的关联。方法采用病例-对照方法 ,应用聚合酶链反应-序列特异性引物(PCR-SSP)技术对226例新疆维吾尔族肺结核病患者(肺结核病例组)和当地同族231人健康者进行HLA-DRB1基因分型,比较其等位基因频率(GF),并计算其比值比(OR)。结果肺结核病例组中HLA-DRB1*11基因频率显著高于健康对照组,两组的GF分别为4.53%和1.09%,差异有统计学意义(OR=4.388,χ2=9.872,Pc=0.026<0.05);肺结核病例组中HLA-DRB1*04基因频率亦显著高于健康对照组,两组的GF分别为12.77%和8.36%,但P值经过校正后差异无统计学意义(Pc>0.05)。结论 HLA-DRB1*11等位基因与新疆维吾尔族人群结核病呈相关性。

The aim of this study was to explore the association between polymorphism of HLA-DRB1 gene and the susceptibility to pulmonary tuberculosis in population of Uygur Nationality. In this case-control study,HLA-DRB1 genes from 226 PTB cases and 231 controls among Uygur Nationality in Xinjiang area were typed by polymerase chain reaction-sequence specific primers （PCR-SSP）and gene frequency （GF）,then the odds ratio （OR） were calculated and compared. The frequency of HLA-DRB1＊11 gene in pulmonary tuberculosis cases was significantly higher than that in the healthy controls （OR=4.388,χ2=9.872,Pc=0.0260.05）,and their GFs were 4.53% and 1.09% respectively. The frequency of HLA-DRB1＊04 gene in pulmonary tuberculosis cases was also significantly higher than that in the healthy controls and their GFs were 12.77% and 8.36%,but the difference was not statistically significant （Pc0.05）. Results indicate that HLA-DRB1＊11 alleles were evidently correlated to the incidence of tuberculosis disease and DRB1＊11 alleles might be the susceptible genes of tuberculosis disease in population of Xinjiang Uygur Nationality.