摘要
目的比较贝氏柯克斯体感染单核细胞和非感染单核细胞的蛋白谱,发现该柯克斯体感染诱导单核细胞表达蛋白。方法用贝氏柯克斯体I相毒株感染人单核细胞(THP-1),采用等电聚焦和SDS-PAGE双向电泳对感染单核细胞和非感染单核细胞同时做蛋白分离,用专业电泳图谱分析软件发现它们之间的差异表达蛋白,用基质辅助激光解析电离飞行时间质谱仪鉴定差异表达蛋白。结果质谱分析鉴定97个差异表达蛋白,经蛋白氨基酸序列数据库检索和生物信息学分析发现其中15个差异表达蛋白参与调节单核细胞的吞噬、杀菌、细胞凋亡等。结论贝氏柯克斯体侵入单核细胞诱导细胞某些蛋白分子差异表达,增强柯克斯体细胞内入侵、抑制单核细胞的杀菌活性和细胞凋亡,促进贝氏柯克斯体在细胞内生存。
In order to understand how Coxiella burnetii grows well in host cells,the monocytes infected with C. burnetiiwas compared in proteomic study. The protein profiles of C. burnetii infected human monocytes (THP-1) and un-infected THP-1 were prepared by two-dimensional electrophoresis. The differentially expressed proteins were identified by MALDI-TOF-MS. By bioinformation analysis,15 of 97 identified proteins were recognized as proteins associated with regulations of phagocytosis,bactericide and apoptosis of monocytes. These results indicated that the internalization of C. burnetii organisms was enhanced,and the bactericidal activity and apoptosis of monocytes were inhibited after infection of C. burnetii. It is also suggested that the differential expression of these proteins are beneficial for survival and growth of C. burnetii in the host cells.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2010年第5期421-424,共4页
Chinese Journal of Zoonoses
基金
国家自然科学基金项目(No.30670101)资助