云南大理白族带绦虫mtDNA-Cytb序列测定及分析

Sequencing and analysis on mtDNA Cytb of Taenia cestodes in Bai population(Dali) of Yunnan province

目的利用分子遗传标记对采自云南大理白族地区人体带绦虫标本进行生物多态性的研究。方法从云南大理采集人体带绦虫标本株成虫节片,抽提虫体基因组DNA,PCR扩增线粒体DNA细胞色素B(mtDNA-Cytb)序列部分片段,测序;结合GenBank中已知猪带绦虫、牛带绦虫和亚洲带绦虫mtDNA-Cytb序列,经DNAMAN软件处理后计算遗传距离并构建系统发育树状图。结果大理白族带绦虫标本系统发育树显示大理(B1,B2,B5,B6)株带绦虫标本与亚洲带绦虫的遗传距离最接近,距牛带绦虫标本较远,与猪带绦虫则更远。大理(B3,B4)株带绦虫标本与猪带绦虫的遗传距离最接近,距牛带绦虫和亚洲带绦虫标本远。结论云南大理(B1,B2,B5,B6)株带绦虫标本属于亚洲带绦虫,而(B3,B4)株带绦虫标本属于猪带绦虫;mtDNA-Cytb序列分析可以用于带绦虫生物多态性研究。

To study the biologic polymorphism of Taenia cestodes samples from Bai populations （Dali） in Yunnan by molecular genetic marker,mitochondrial DNA were extracted from the proglottis of adult Taenia cestodes strains and partial segments of the mitochondrial DNA Cytochrome B （mtDNA Cytb） sequence were amplified by polymerase chain reaction （PCR）. Phylogenetic tree of species and geographical strains was constructed with the given mtDNA Cytb of T. solium,T. saginata and T. saginata asiatica in GenBank by DNAMAN software. The phylogenetic tree revealed that the Taenia cestodes samples （B1,B2,B5,B6） from Bai populations （Dali） in Yunnan province were close to Asia strain genetically,while they were far from T. saginata and even far from T. solium. Samples（B3,B4）were closed to T. solium but far from T. saginata and Taenia cestodes in Asia. It was apparent that the Taenia cestodes samples （B1,B2,B5,B6） from Bai populations （Dali） in Yunnan province belonged to Taenia cestodes in Asia and（B3,B4）belonged to T. solium. It also suggested that mtDNA Cytb sequence analysis could be applied to the study on biologic polymorphism of Taenia cestodes.