Survivin和Smac在卵巢黏液性肿瘤中的表达及意义

Expression and significance of Survivin and Smac in ovarian mutinous tumors

目的 探讨Survivin和Smae在卵巢黏液性肿瘤中的表达及意义.方法 应用免疫组织化学SABC法检测55例原发性卵巢黏液性肿瘤中的Survivin和Smac表达,并应用免疫电镜胶体金标记法观察Smac在原发性卵巢黏液性肿瘤中的亚细胞定位及表达趋势.结果 （1）Survivin在良性、交界性和恶性组的阳性表达率分别为2/20、12/15和20/20,呈升高趋势,其中良性与交界性、良性与恶性组间比较差异均有统计学意义（P〈0.01）.良性、交界性和恶性组Survivin的胞核阳性表达率分别为1/20、6/15和5/20,其中良性与交界性组比较差异有统计学意义（P〈0.05）.Smae在良性、交界性和恶性组的阳性表达率分别为19/20、9/15和3/20,呈降低趋势,三组间两两比较差异均有统计学意义（P〈0.01或〈0.05）.Survivin和Smac之间呈负相关（r=-0.153,P〈0.01）.（2）良性、交界性和恶性组线粒体内Smae金标颗粒计数分别为24.1±7.2、11.1±1.9和5.2±1.7,三组间两两比较差异均有统计学意义（P〈0.01或〈0.05）;线粒体外计数分别为4.7±3.0、2.9±1.0和1.7±1.3,三组间比较差异无统计学意义（P〉0.05）.结论 随着卵巢黏液性肿瘤的恶性进展,凋亡抑制蛋白Survivin表达上调,促凋亡蛋白Smac表达下调;下调的Smac蛋白表达主要为储存在线粒体膜间的非活性形式蛋白,而非释放到胞质中的活性形式蛋白.

Objective To investigate the expressions and significances of Survivin and Smac in ovarian mucinous tumors. Methods A total of 55 paraffin-embedded specimens of primary ovarian mucinous tumors were collected. SABC was used to detect protein expression of Survivin and Smac genes.Immunoelectron microscopy using colloidal gold labeling was performed to determine the subcellular localization and patterns of Smac protein expression. Results （1） The cytoplasmic expression rates of survivin in benign, borderline and malignant ovarian mucinous tumors were 2/20, 12/15 and 20/20 respectively, which presents an improving trend. There were significant differences of survivin expression between benign vs. Borderline lesions （P 〈 0.01） , and benign vs. Malignant tumors （P 〈 0. 01） . The nuclear expression rates of survivin in benign, borderline and malignant ovarian mucinous tumors were 1/20,6/15 and 5/20, respectively, which presents a. Declining trend. There was significant difference of survivin expression between benign vs. Borderline tumors （P 〈 0. 05）. The positive expression rates of Smac among the three groups were 19/20, 9/15 and 3/20, respectively. There was significant difference among the three groups （P〈0. 01 or 〈0.05）. There was a negative correlation between Survivin and Smac （r= -0. 153,P 〈0. 01）. （2） Colloidal gold labeling study demonstrated that mitochondrion intramembranous storage of Smac granules in the three groups were 24. 1 ±7.2, 11. 1 ±1.9 and 5. 2±1. 7, respectively, and there were significant differences among the three groups （P 〈 0. 01 or 〈 0.05）. The extramemebranous Smac granules were 4. 7±3. 0, 2. 9±1. 0 and 1. 7 ±1. 3, although without significant difference among the three groups （P 〉0. 05）. Conclusions With the malignant development of ovarian mucinous tumors,the expressions of Survivin are up-regulated,and the expressions of Smac are down-regulated. Smac proteins exist mainly in an inactive intramembranous storage form inside of mitochondria.