Multilayered polyelectrolyte-coated gold nanorods as multifunctional optical contrast agents for cancer cell imaging 被引量：1

Multilayered polyelectrolyte-coated gold nanorods as multifunctional optical contrast agents for cancer cell imaging

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摘要 We report the application of multilayered polyelectrolyte-coated gold nanorods(GNRs) as multifunctional optical contrast agents for cancer cell imaging.The surface modification of GNRs improves their chemical stability and facilitates them to be taken up by cancer cells through electrostatic interaction.The unique longitudinal surface plasmon resonance property of GNRs makes them suitable as both "scattering contrast agents" and "Raman contrast agents".In our experiments,the staining of GNRs in cells was further confirmed by dark field microscopy and Raman microscopy.Our experiment results indicated that GNRs have great potential as multifunctional "optical contrast agents" for future in vivo animal imaging. We report the application of multilayered polyelectrolyte-coated gold nanorods（GNRs） as multifunctional optical contrast agents for cancer cell imaging.The surface modification of GNRs improves their chemical stability and facilitates them to be taken up by cancer cells through electrostatic interaction.The unique longitudinal surface plasmon resonance property of GNRs makes them suitable as both ＂scattering contrast agents＂ and ＂Raman contrast agents＂.In our experiments,the staining of GNRs in cells was further confirmed by dark field microscopy and Raman microscopy.Our experiment results indicated that GNRs have great potential as multifunctional ＂optical contrast agents＂ for future in vivo animal imaging.

作者 Li-li CHEN Li JIANG Ya-lun WANG Jun QIAN Sailing HE

机构地区 Centre for Optical and Electromagnetic Research Department of Electromagnetic Engineering

出处《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第6期417-422,共6页 浙江大学学报（英文版）B辑（生物医学与生物技术）

基金 supported by the Swedish Foundation for Strategic Research (SSF) and the US Air Force Office of Scientific Research (Asia Office)

关键词 Gold nanorods POLYELECTROLYTE Scattering RAMAN Cancer cell imaging 关键词金牌 nanorods；聚合电解质；散布；拉曼；癌症房间成像

分类号 Q2 [生物学—细胞生物学] TN2 [电子电信—物理电子学]

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1Camden, J.P., Dieringer, J.A., Wang, Y.M., Masiello, D.J., Marks, L.D., Schatz, G.C., Van-Duyne, R.P., 2008. Probing the structure of single-molecule surface- enhanced Raman scattering hot spots. Journal of the American Chemical Society, 130(38): 12616-12617. [doi: 10.1021/ja8051427].
2Ding, H., Yong, K.T., Roy, I., Pudawar, H.E., Law, W.C., Bergey, E.J., Prasad, P.N., 2007. Gold nanorods coated with multilayer polyelectrolyte as contrast agents for multimodal imaging. Journal of Physical Chemistry C, 111(34): 12552-12557. [doi:10.1021/jp0733419].
3Hu, R., Yong, K., Roy, I., Ding, H., He, S., Prasad, P.N., 2009. Metallic nanostructures as localized plasmon resonance enhanced scattering probes for multiplex dark-field targeted imaging of cancer cells. The Journal of Physical Chemistry C, 113(7):2676-2684. [doi:10.1021/jp8076672].
4Huang, X.H., El-Sayed, I.H., Qian, W., El-Sayed, M.A., 2006. Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. Journal of the American Chemical Society, 128(6):2115-2120. [doi:10.1021/ja057254a].
5Huang, X.H., El-Sayed, I.H., Qian, W., El-Sayed, M.A., 2007. Cancer cells assemble and align gold nanorods conjugated to antibodies to produce highly enhanced, sharp, and polarized surface raman spectra: a potential cancer diagnostic marker. Nano Letters, 7(6): 1591-1597. [doi:10.1021/n1070472c].
6Jain, P.K., Lee, K.S., El-Sayed, LH., El-Sayed, M.A., 2006. Calculated absorption and scattering properties of gold nanoparticles of different size, shape, and composition: Applications in biological imaging and biomedicine. Journal of Physical Chemistry B, 110(14):7238-7248. [doi:10.1021/jp057170o].
7Kim, S., Jung, Y., Gu, G.H., Suh, J.S., Park, S.M., Ryu, S., 2009. Discrete dipole approximation calculations of optical properties of silver nanorod arrays in porous anodic alumina. Journal of Physical Chemistry C, 113(37): 16321-16328. [doi:10.1021/jp811516s].
8Lee, S.E., Sasaki, D.Y., Perroud, T.D., Yoo, D., Patel, K.D., Lee, L.P., 2009. Biologically functional cationic phospholipid-gold nanoplasmonic carriers of RNA. Journal of the American Chemical Society, 131(39): 14066-14074. [doi:10.1021/ja904326j].
9Li, X., Qian, J., He, S.L., 2008. Impact of the self-assembly of multilayer polyelectrolyte functionalized gold nanorods and its application to biosensing. Nanotechnology, 19(35): 355501-355507. [doi:10.1088/0957-4484/19/35/355501].
10Link, S., Mohamed, M.B., El-Sayed, M.A., 1999. Simulation of the optical absorption spectra of gold nanorods as a function of their aspect ratio and the effect of the medium dielectric constant. Journal of Physical Chemistry B, 103(16):3073-3077. [doi:10.1021/jp990183f].