摘要
根据GenBank中登录的大熊猫轮状病毒(RV)VP7基因序列,设计了1对特异性引物,建立了快速检测大熊猫RV的RT-PCR方法。用该方法对MA-104细胞增殖的大熊猫RV进行RT-PCR扩增,结果得到与试验设计相符的342bp的特异性扩增条带,而对传染性胃肠炎病毒、大肠杆菌和沙门氏菌的扩增结果为阴性。测序比对结果证实该体系检测结果准确;该方法最低可检测出约1pg的病毒核酸;重复性试验结果显示,其检测重复性好,提示该RT-PCR方法可用于大熊猫RV的临床快速检测。
Based on the published sequence of the giant panda rotavirus strain CH-1 VP7 gene in GenBank,a pair of specific primers was designed,and a rapid RT-PCR method was established for detecting rotavirus in giant panda.The specific band of 342bp was amplified from the culture of MA-104,but no speci-fic band was amplified from transmissible gastroenteritis virus,Escherichia coli and Salmonella.Sequence analysis indicated that this method was accurate.As little as 1pg RNA of giant panda rotavirus could be detected,and repeatability was good.In conclusion,the established RT-PCR method could be used for the detection of rotavirus in giant panda.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2010年第5期490-493,共4页
Chinese Veterinary Science
基金
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)
成都大熊猫繁育研究基金会项目(CPF08013)