兔骨关节炎滑膜组织血管内皮生长因子mRNA表达及透明质酸钠的影响

Effect of sodium hyaluronateon on vascular endothelial growth factor mRNA expression of the synovium in rabbits with osteoarthritis

背景:骨关节炎的发病机制目前还未完全清楚,研究发现血管内皮生长因子参与骨关节炎的发生发展。目的:观察血管内皮生长因子在滑膜组织表达的改变及透明质酸钠对滑膜血管内皮生长因子基因表达的影响。方法:24只大耳白兔随机正常对照组、生理盐水组和透明质酸钠组行单膝前交叉韧带切断术,4周后生理盐水组关节腔注射生理盐水0.3mL,透明质酸钠组注射等量的高分子量10g/L透明质酸钠,1次/周,连续5周。第10周处死动物,比较各组股骨内髁关节软骨的大体变化,采用实时定量聚合酶链反应方法检测滑膜VEGFmRNA的表达水平。结果与结论:大体评分显示生理盐水组软骨退变的程度明显重于正常对照组和透明质酸钠组(P<0.05);生理盐水组滑膜血管内皮生长因子mRNA表达较正常组显著下降(P<0.05),透明质酸钠组滑膜血管内皮生长因子mRNA表达较生理盐水组有升高,但比正常对照组低(P<0.05)。结果表明,滑膜组织血管内皮生长因子表达下降可能参与透明质酸钠的发生发展,关节腔注射透明质酸钠对透明质酸钠滑膜组织血管内皮生长因子表达有上调作用,有利于滑膜组织修复,可能是其治疗透明质酸钠机制之一。

BACKGROUND： Pathogenesis of osteoarthritis is poorly understood, however, studies have demonstrated that vascular endothelial growth factor （VEGF） is involved in the progression of osteoarthritis. OBJECTIVE： To detect the changes of VEGF mRNA expression of synovium in rabbit osteoarthritis and to evaluate the effect of sodium hyaluronate on its expression. METHODS： Twenty four white rabbits were divided into the normal control, physiologic saline, and sodium hyaluronate groups. The unilateral anterior cruciate ligament transection （ACLT） was performed in the physiologic saline and sodium hyaluronate groups. At weeks 4 after operation, rabbits in the physiologic saline group were injected 0.3 mL physiologic saline, and in the sodium hyaluronate group received 10 g/L sodium hyaluronate injection, once per week for 5 successive weeks. All the animals were sacrificed at week 10 after operation. The cartilage changes on the medial femoral condyles were graded separately. VEGF expression of synovium was detected by using real time polymerase chain reaction （RT-PCR）. RESULTS AND CONCLUSION： The macroscopic score showed that the cartilage degeneration in the physiologic saline group was significantly more severe than that of the normal control and sodium hyaluronate groups （P 0.05）. The expression of VEGF mRNA was obviously decreased in the physiologic saline group than that of the normal control group （P 0.05）, of which was increased in thesodium hyaluronate group, but still smaller than the normal control group （P 0.05）. The results demonstrated that the decreased VEGF expression in synovium may involved in the progression of osteoarthritis, and sodium hyaluronate has protective effect on articular cartilage by up-regulating the VEGF expression.