小鼠胚胎成纤维细胞成熟化过程中肿瘤坏死因子α的作用(英文)

Effects of tumor necrosis factor-alpha on maturity of mouse embryonic fibroblasts

背景:近年的研究表明,肿瘤坏死因子α对不同组织成纤维细胞的作用具有组织特异性及浓度依赖性。目的:观察肿瘤坏死因子α及其信号传导途径中特异性激酶抑制剂对小鼠胚胎成纤维细胞成熟化所起的作用。方法:体外培养小鼠胚胎成纤维细胞,将细胞分为3组:第1组用含体积分数2%血清的DMEM高糖培养基培养作为空白对照组;第2组用含100μg/L肿瘤坏死因子α的培养基培养;第3组先加入质量浓度为50μg/L的Anti-TNFRSF1B作用1h后,倒出培养基再加入含有肿瘤坏死因子α的培养基继续培养。采用RT-PCR法测定各组Ⅰ型胶原蛋白和基质金属蛋白酶3mRNA表达、Western Blot法测定各组Ⅰ型胶原蛋白和基质金属蛋白酶3蛋白表达。结果与结论:小鼠胚胎成纤维细胞在一定质量浓度肿瘤坏死因子α作用下,其信号传导途径特异性激酶发生磷酸化或蛋白被激活,信号通路被激活,促进基质金属蛋白酶3的活化,明显降低Ⅰ型胶原的表达。加入其信号传导途径的抑制剂Anti-TNFRSF1B后,肿瘤坏死因子α的效应得到了一定的抑制,但并未完全消除,这更进一步证明肿瘤坏死因子α对小鼠胚胎成纤维细胞活化的作用。

BACKGROUND： In recent years, studies have shown the effects of tumor necrosis factor-α （TNF-α） on fibroblasts at different organizations in a tissue-specific and dose-dependent manner. OBJECTIVE： To investigate the biologic effects of TNF-α and its signal transduction pathway-specific kinase inhibitors on mouse embryonic fibroblasts （NIH3T3）. METHODS： NIH3T3 cells were cultured in vitro, and then the cells were assigned into 3 groups. Cells in the control group were cultured in DMEM high-glucose medium with 2% serum; those in the TNF-α group were cultured in 100 μg/L TNF-α medium; those in the TNF-α＋Anti-TNFRSF1B group were firstly cultured in medium with 50 μg/L Anti-TNFRSF1B for 1 hour, and then placed in the medium with 100 μg/L TNF-α. RT-PCR and Western blot methods were used to evaluate mRNA and protein expressions of type Ⅰ collagen and matrix metalloproteinase 3 （MMP3） in each group. RESULTS AND CONCLUSION： In this experiment, NIH3T3 cells cultured with a certain concentration of TNF-α, the specificity kinase signal of transduction pathway presented with phosphorylation or protein activation, and the signal pathway was activated, which promoted MMP3 activation, and significantly reduced the expression of type Ⅰ collagen. The effect of TNF-α was certainly inhibited, but not completely eliminated after adding its signal transduction pathway inhibitor Anti-TNFRSF1B. This further proves the role of TNF-α on NIH3T3 activation.